摘要

   来自加药牲畜的粪便含有药物抗生素和抗生素抗性基因 (ARG)。生物可利用的抗生素在粪便储存期间触发进一步的 ARG 扩增。测试了 biochar 是否降低了粪便中抗生素磺胺二甲嘧啶 (SMZ)、环丙沙星 (CIP)、土霉素 (OTC) 和氟苯尼考 (FF) 的生物利用度以及 sul1 和 tet(W) ARGs 的扩增。为此，用 5% (w/w) 松果生物炭 (BCP) 处理液态猪粪。抗生素在 30 天的孵育过程中以 SMZ < OTC < CIP < FF 的顺序消散。添加 BCP 进一步固定了 SMZ、OTC 和 CIP，而对 FF 的影响不显着。在 OTC 和 SMZ 加标粪便中，sul1 和 tet(W) ARGs 拷贝数分别显着增加了 5.8 和 2.5 倍。 BCP改良粪肥中sul1的丰度显着降低，而对tet(W)的影响较小。因此，生物炭适用于储存期间被抗生素污染的粪便的管理。

  Manure from medicated livestock contains pharmaceutical antibiotics and antibiotic resistance genes (ARGs). Bioavailable antibiotics trigger further ARGs amplification during manure storage. It was tested whether biochar lowers the bioavailability of the antibiotics sulfamethazine (SMZ), ciprofloxacin (CIP), oxytetracycline (OTC) and florfenicol (FF) in manure and the amplification of sul1 and tet(W) ARGs. To that end, liquid pig manure was treated with 5% (w/w) pinecone biochar (BCP). Antibiotics dissipated during 30-d incubation in the order SMZ < OTC < CIP < FF. Added BCP further immobilized SMZ, OTC and CIP, while the effect was not significant for FF. Both sul1 and tet(W) ARGs copy numbers significantly increased by factors of 5.8 and 2.5, respectively, in OTC and SMZ spiked manure. The abundance of sul1 was significantly decreased in BCP amended manure, while the impact on tet(W) was less. Consequently, biochar is suitable for the management of antibiotics contaminated manure during storage.

  https://www.sciencedirect.com/science/article/abs/pii/S0960852420310543