摘要

  在这项研究中，研究了在猪粪厌氧消化 (AD) 过程中添加氯化亚铁 (FeCl2) 对甲烷产生和抗生素抗性基因 (ARG) 减少的影响。 FeCl2 既可以提高累积甲烷产量，也可以降低总 ARGs 的丰度，即在 FC5 处 CH4 产量最大增加 21.5%，在 FC25 处最大 ARGs 减少 33.3%。 病原菌和金属抗性基因（MRGs）的减少得到加强。 通过提高 H2 利用率和直接种间电子转移 (DIET)，乙酸盐和丙酸盐的利用率得到加强，其中 FeCl2 和乙酸的反应进一步增强了 DIET。 细菌群落在ARGs（68.26%）的进化中起重要作用，其也受MRGs、移动遗传元件（MGEs）和环境因素的影响。 因此，基于 FeCl2 的 AD 是一种提高甲烷产量和减少 ARG 的可行且有吸引力的方法。

   In this study, effects of ferrous chloride (FeCl2) addition on methane production and antibiotic resistance genes (ARGs) reduction were investigated during anaerobic digestion (AD) of swine manure. FeCl2 could both improve the accumulative methane production and reduce the abundance of total ARGs, i.e., the maximum increase of CH4 production of 21.5% at FC5, and the maximum ARGs reduction of 33.3% at FC25. The reduction of pathogenic bacteria and metal resistance genes (MRGs) was enhanced. Acetate and propionate utilization were intensified by enhancing H2 utilization and direct interspecies electron transfer (DIET), where DIET was further enhanced by the reaction of the FeCl2 and acetic acid. The bacterial community played important role in the evolution of ARGs (68.26%), which were also affected by MRGs, mobile genetic elements (MGEs), and environmental factors. Therefore, FeCl2-based AD is a feasible and attractive way to improve methane production and ARG reduction.

  https://www.sciencedirect.com/science/article/abs/pii/S0960852419317493