摘要

      需要研究来描述不同抗生素施用和粪便管理实践在放大或减弱抗生素耐药性传播潜力方面的相对和综合影响。在这里，我们对标准抗生素施用（头孢匹林/吡利霉素或磺胺二甲嘧啶/金霉素/泰乐菌素）与未经处理的牛相比，在“抗性组”（通过鸟枪宏基因组测序确定的总抗生素抗性基因 (ARG)）、细菌微生物群和通过定量聚合酶链反应计数的指示剂 ARG 上。为了深入了解嗜热相的作用，堆肥还被外部加热至 > 55 °C × 15 天。

     Research is needed to delineate the relative and combined effects of different antibiotic administration and manure management practices in either amplifying or attenuating the potential for antibiotic resistance to spread. Here, we carried out a comprehensive parallel examination of the effects of small-scale (> 55 °C × 3 days) static and turned composting of manures from dairy and beef cattle collected during standard antibiotic administration (cephapirin/pirlimycin or sulfamethazine/chlortetracycline/tylosin, respectively), versus from untreated cattle, on “resistomes” (total antibiotic resistance genes (ARGs) determined via shotgun metagenomic sequencing), bacterial microbiota, and indicator ARGs enumerated via quantitative polymerase chain reaction. To gain insight into the role of the thermophilic phase, compost was also externally heated to > 55 °C × 15 days.

https://link.springer.com/article/10.1186/s40168-021-01006-z