摘要

      抗生素抗性基因 (ARG) 在环境中的传播导致全球抗生素抗性感染的增加。 因此，进一步研究这些新兴污染物对人类的暴露途径具有重要意义。 本研究在对 34 种园林产品、3 种近期美化土壤和 5 种原生土壤的调查中探索了含有动物粪便的市售园林产品作为 ARG 的来源。 从这些土壤中提取 DNA 并量化 5 个 ARG、intI1 和 16S rRNA。 这项研究发现，园艺产品中的绝对和相对基因丰度比在天然土壤中观察到的高约 2 到 4 个数量级。 获得有机材料审查研究所 (OMRI) 认证的园艺产品的 ARG 丰度没有显着差异。 这里的结果表明，花园产品是花园、草坪和公园 ARG 的重要来源。

      The dissemination of antibiotic resistance genes (ARGs) in the environment contributes to the global rise in antibiotic resistant infections. Therefore, it is of importance to further research the exposure pathways of these emerging contaminants to humans. This study explores commercially available garden products containing animal manure as a source of ARGs in a survey of 34 garden products, 3 recently landscaped soils, and 5 native soils. DNA was extracted from these soils and quantified for 5 ARGs, intI1, and 16S rRNA. This study found that both absolute and relative gene abundances in garden products ranged from approximately two to greater than four orders of magnitude higher than those observed in native soils. Garden products with Organic Materials Review Institute (OMRI) certification did not have significantly different ARG abundances. Results here indicate that garden products are important sources of ARGs to gardens, lawns, and parks.

https://link.springer.com/article/10.1007/s11356-021-13333-7