摘要

       量化南极耐抗生素细菌 (ARB) 的出现对于评估污染水平和评估人类无人居住环境中 ARB 的“基线”或背景水平至关重要。从菲尔德斯半岛采集了动物粪便、土壤和沉积物的样本。研究了 ARB 中磺胺二甲嘧啶和环丙沙星抗性细菌和抗生素抗性基因 (ARG) 的丰度。结果表明，从样品中分离出的环丙沙星和磺胺二甲嘧啶耐药菌的丰度最高，分别为 30 CFU/g 和 79.8 CFU/g。磺胺二甲嘧啶和喹诺酮耐药菌的优势属分别为假单胞菌和节杆菌。从 ARB 中检测到 106 个 ARG。发现了移动遗传元件（MGEs）和 ARGs 之间的强正相关，这是一个相对新颖的观察结果，该机制被证实也发生在南极。本研究揭示了南极菌株ARGs的组成特征，为南极抗生素耐药性的来源和耐药机制提供了支持。

       Quantifying the occurrence of Antarctic antibiotic-resistant bacteria (ARB) is essential for assessing the level of pollution and assessing the “baseline” or background level of ARB in human uninhabited environments. Animal feces, soil, and sediments were sampled from Fildes Peninsula. The abundance of sulfamethazine- and ciprofloxacin-resistance bacteria and antibotic resistance genes (ARGs) within ARB were investigated. The results showed Ciprofloxacin- and Sulfamethazine-resistant bacteria isolated from samples accounted for the highest abundances of 30 CFU/g and 79.8 CFU/g, respectively. The dominant genus of Sulfamethazine-and quinolone-resistance bacteria was Pseudomonas and Arthrobacter, respectively. 106 ARGs were detected from ARB. Strong positive correlations between mobile genetic elements (MGEs) and ARGs were found, what is relatively novel observation that the mechanism is confirmed to also occur in the Antarctic. This study reveals the compositional characteristics of ARGs of strains in Antarctic, providing support for the source of Antarctic antibiotic resistance and drug resistance mechanisms.

https://www.sciencedirect.com/science/article/pii/S0025326X20309474