摘要

       据报道，单一的好氧或厌氧堆肥对于从动物粪便中去除抗生素抗性基因 (ARG) 效率低下。本研究调查了在不同条件下有氧联合厌氧堆肥 (ACA) 过程中 ARG 去除的影响。结果表明，每天翻动一次、猪粪与玉米秸秆的比例（3:1）、厌氧时间为6天是降低ARG（>95%，尤其是ermF：99.78%）的最佳操作条件。 ) 在 ACA 期间。此外，冗余分析表明细菌群落（特别是 Truepera、Petrimonas 和 Ureibacillus）对 ARG 去除的影响比环境因素（特别是温度、总磷、氨氮和 pH 值）更强。网络分析表明，这些潜在宿主细菌通过个体 ARGs 和特定细菌之间的显着共存对 ARGs 的传播产生了重要影响。这些发现提供了一种有效的技术来减少和阻止从动物粪便中传播的 ARG。

       Single aerobic or anaerobic composting is reportedly as inefficient for removing antibiotic resistance genes (ARGs) from animal manure. This study investigates the effects of ARG removal during aerobic combined with anaerobic composting (ACA) under different conditions. The results showed that a turning frequency of once a day, the proportion of swine manure and corn straw (3:1) and an anaerobic time of 6 days were the best operating conditions for ARG reduction (>95%, especially ermF: 99.78%) during ACA. Moreover, redundancy analysis demonstrated that the bacterial community (especially Truepera, Petrimonas and Ureibacillus) had a stronger effect on ARG removal than environmental factors did (especially temperature, total phosphorus, ammonia nitrogen and pH). Network analysis indicated the important effect of these potential host bacteria on the spread of ARGs through significant co-occurrence between individual ARGs and specific bacteria. These findings offer an effective technology to reduce and block ARGs spread from animal manure.

https://www.sciencedirect.com/science/article/abs/pii/S0960852421000481