摘要

       本研究比较了添加 0% (CK)、5% (L)、10% (M) 和 15% (H) 四个水平的虾壳粉 (SSP) 对抗生素抗性基因丰度的影响。 ARGs）和猪粪堆肥过程中的细菌群落演替。 CK 中 5/11 ARGs 的相对丰度降低，H 中为 7/11。此外，添加 SSP 提高了去除率。因此，H 使 ARG 的总丰度降低了 32.68%，而 CK 增加了 6.31%。冗余分析表明，移动遗传元件（MGEs）（46.6%）和细菌群落（31.1%）主要解释了ARGs的变化。 H 增强了 MGEs 的去除，延长了高温相，稳定了铜和锌，并保留了氮。 LEfSe 分析和非度量多维标度表明，堆肥过程中细菌群落发生了变化，并被 H 优化。 ARGs 和 MGEs 的潜在细菌共宿主（如乳酸杆菌、棒状杆菌_1 和鸟氨酸链球菌）的丰度分别为与 CK 相比，H 中的有机物分解率更高。因此，用 15% SSP 进行堆肥可以降低 ARGs 的风险，提高农艺应用的实用价值。

       The present study compared the effects of adding shrimp shell powder (SSP) at four levels comprising 0% (CK), 5% (L), 10% (M), and 15% (H) on the abundance of antibiotic resistance genes (ARGs) and the bacterial community succession during swine manure composting. The relative abundances of 5/11 ARGs were reduced in CK, and 7/11 in H. Moreover, the removal rate was enhanced by adding SSP. Thus, H decreased the total abundance of ARGs by 32.68%, whereas CK increased it by 6.31%. Redundancy analysis indicated that mobile genetic elements (MGEs) (46.6%) and the bacterial community (31.1%) mainly explained the changes in ARGs. H enhanced the removal of MGEs, prolonged the thermophilic phase, stabilized copper and zinc, and retained nitrogen. LEfSe analysis and non-metric multidimensional scaling indicated that the bacterial community changed in the composting process, and it was optimized by H. The abundances of the potential bacterial co-hosts (such as Lactobacillus, Corynebacterium_1, and Ornithinicoccus) of ARGs and MGEs were lower and the decomposition of organic matter was higher in H compared with CK. Thus, composting with 15% SSP can reduce the risk of ARGs and improve the practical value for agronomic application.

https://www.sciencedirect.com/science/article/abs/pii/S0048969720356916