摘要

       抗生素耐药性在世界范围内受到越来越多的关注，污水处理厂已被视为抗生素耐药细菌和抗生素耐药基因（ARGs）的热点。在这项研究中，我们评估了通过超声 (US) 处理与不同输入水平的 US 特定能量结合紫外发光二极管 (UV-LED) 去除四环素抗性大肠杆菌及其相关基因的情况。在正常建议的 UV-LED 剂量（低于 30 mJ/cm2）下，同时使用 UV-LED 的 US 有效地消除了耐四环素的大肠杆菌。去除效率随着 US 的加入而增加（比输入能量为 8-16 kJ/L），同时使用 UV-LED 进行 US 处理比 US 预处理相对更有效。 K+ 泄漏和流式细胞术对细胞损伤的分析表明，细胞壁在应用的处理条件下保持其完整性。因此，16 S rRNA、tet M 和 tet Q 的去除效率并不令人满意，因为实现的对数减少少于 1。增加美国能量会显着破坏细胞壁并可能促进反应。与单独使用 UV-LED 相比，当使用 US 特定输入能量 330 kJ/L 和 5 mJ/cm2 时，ARG 的去除增加了四倍。结合 UV-LED 的美国处理是一种不需要化学品的新工艺。本研究结果可为ARGs的去除提供理论支持。

       Antibiotic resistance has gained increasing attention worldwide, and wastewater treatment plants have been regarded as hotspots for antibiotic-resistant bacteria and antibiotic-resistant genes (ARGs). In this study, we evaluated the removal of tetracycline-resistant Escherichia coli and its related genes through ultrasound (US) treatment with different input levels of US-specific energy combined with ultraviolet light emitting diodes (UV-LEDs). Simultaneous US with UV-LEDs effectively eliminated tetracycline-resistant E. coli with the normal suggested UV-LEDs dosage (below 30 mJ/cm2). The removal efficiency increased with the addition of US (specific input energy of 8–16 kJ/L), and simultaneous US treatment with UV-LEDs was relatively more effective than US pretreatment. Analyses of cell damage by K+ leakage and flow cytometry showed that the cell wall kept its integrity during the applied treatment conditions. Consequently, the removal efficiencies of 16 S rRNA, tet M, and tet Q were unsatisfactory because less than 1 log reduction was achieved. Increasing the US energy remarkably damaged the cell wall and potentially promoted the reaction. The removal of ARGs increased four times when using US-specific input energy at 330 kJ/L with 5 mJ/cm2 compared with UV-LEDs alone. The US treatment combined with UV-LEDs is a novel process that does not require chemicals. Results of this research can provide theoretical support for the removal of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0013935121003017