摘要

       饮用水中对抗生素和重金属的多重耐药性对人类健康构成重大威胁。 在此，我们利用 qPCR 来评估中国河南省四个城市的井水和自来水样品中抗生素抗性基因 (ARGs)、重金属抗性基因 (HMRGs) 和 1 类整合子 (intI1) 基因表达水平的模式。 大部分指标值的相对丰度在井水中高于自来水，或平均而言商丘市最高，濮阳市最低。 井水和自来水中ARG的表达与intI1和HMRG的表达密切相关。 总体而言，我们的数据突出了饮用水供应中 ARG 构成的健康威胁，并强调了在与人类活动和重金属压力相关的选择压力下，借助 intI1，这些基因在细菌之间转移的潜力。 

       The presence of multi-resistance to both antibiotics and heavy metals in drinking water poses a significant risk to human health. Herein, we utilized qPCR to assess patterns of antibiotic resistance genes (ARGs), heavy metal resistance genes (HMRGs), and class 1 integron (intI1) gene expression levels in well and tap water samples from four cities in Henan Province, China. The relative abundance of most index values was higher in well water relative to tap water, or was highest in Shangqiu City and lowest in Puyang City on average. The expression of ARG was closely correlated with that of intI1 and HMRG in both well and tap water. Overall, our data highlighted the health threat posed by ARGs in the drinking water supply and underscore the potential for the transfer of these genes between bacteria with the aid of intI1 under selective pressure associated with human activity and heavy metal stress.

https://link.springer.com/article/10.1007/s00128-021-03144-4