摘要

       在这项研究中，我们评估了从西西里岛西北部沿海地区的溪流和海水中同时收集的水和聚乙烯 (PE) 废物碎片的微生物组和抵抗组谱。 尽管通过对细菌 16S rDNA 基因的 V3-V4 区域进行测序确定了核心微生物组，但在 PE 废物和相应水样上的微生物群落之间发现了数量差异。 我们的研究结果表明，与相应的水样相比，PE 废物包含更丰富和更多的核心微生物群多样性。 此外，特定抗生素抗性基因 (ARG) 的 PCR 分析表明，PE 废物比水样含有更多的 ARG。 因此，PE 废物可以作为耐抗生素微生物群的载体，也代表对海洋环境和生物体的危险增加。

       In this study, we evaluated the microbiome and the resistome profile of water and fragments of polyethylene (PE) waste collected at the same time from a stream and the seawater in a coastal area of Northwestern Sicily. Although a core microbiome was determined by sequencing of the V3–V4 region of the bacterial 16S rDNA gene, quantitative differences were found among the microbial communities on PE waste and the corresponding water samples. Our findings indicated that PE waste contains a more abundant and increased core microbiome diversity than the corresponding water samples. Moreover, PCR analysis of specific antibiotic resistance genes (ARGs) showed that PE waste harbors more ARGs than the water samples. Thus, PE waste could act as a carrier of antibiotic-resistant microbiota, representing an increased danger for the marine environment and living organisms, as well. View Full-Text

https://www.mdpi.com/2079-7737/10/3/200