摘要

       本研究旨在确定产碳青霉烯酶铜绿假单胞菌分离株的全球分布和分子特征。截至 2019 年 10 月，共从公共数据库中获得了 328 株（11.1%，328/2953）产碳青霉烯酶的人类铜绿假单胞菌菌株。其中，blaVIM 和 blaIMP 基因是铜绿假单胞菌菌株中最普遍的碳青霉烯酶.这些产碳青霉烯酶的铜绿假单胞菌分离株具有 34 种不同的序列类型 (ST)，其中 6 种占优势：ST357、ST823、ST308、ST233、ST175 和 ST111。 ST357 和 ST823 分离株主要在亚洲发现，所有 ST175 分离株都在欧洲发现。 ST308、ST233 和 ST111 分离株在世界范围内传播。此外，所有 ST823 分离株和大多数 ST111、ST233 和 ST175 分离株都携带 blaVIM，但 ST357 分离株主要携带 blaIMP。 ST308 分离株为 blaVIM、blaIMP 和 blaNDM 的传播提供了一个关键的储存库。 WGS分析显示ST111携带的ARG类型非常多样（n = 23），其次是ST357（n = 21）、ST308（n = 19）、ST233（n = 18）、ST175（n = 14）和ST823（ n = 10）。 ST175 分离株携带更多多样性和频繁的氨基糖苷 ARG，ST233 分离株携带更多四环素 ARG。我们的研究结果表明，不同的碳青霉烯类抗性基因主要分布在铜绿假单胞菌分离株的变异 ST 中，这些分离株还具有广泛的地理分布，这突出了监测研究以检测人类中产碳青霉烯酶的铜绿假单胞菌分离株的必要性。

      This study aimed to determine the global distribution and molecular characteristics of carbapenemase-producing Pseudomonas aeruginosa isolates. A total of 328 (11.1%, 328/2953) carbapenemase-producing P. aeruginosa isolates from humans were obtained from public databases as of October 2019. Of which, the blaVIM and blaIMP genes were the most prevalent carbapenemases in the P. aeruginosa isolates. These carbapenemase-producing P. aeruginosa isolates possessed 34 distinct sequence types (STs) and six predominated: ST357, ST823, ST308, ST233, ST175 and ST111. The ST357 and ST823 isolates were primarily found detected in Asia and all ST175 isolates were found in Europe. The ST308, ST233 and ST111 isolates were spread worldwide. Further, all ST823 isolates and the majority of ST111, ST233 and ST175 isolates carried blaVIM but ST357 isolates primarily carried blaIMP. ST308 isolates provide a key reservoir for the spread of blaVIM, blaIMP and blaNDM. WGS analysis revealed that ST111 carried a great diversity of ARG types (n = 23), followed by ST357 (n = 21), ST308 (n = 19), ST233 (n = 18), ST175 (n = 14) and ST823 (n = 10). The ST175 isolates carried a more diversity and frequent of aminoglycoside ARGs, and ST233 isolates harbored more tetracycline ARGs. Our findings revealed that different carbapenem resistance genes were distributed primarily in variant STs of P. aeruginosa isolates, these isolates also possessed an extensive geographical distribution that highlights the need for surveillance studies that detect carbapenemase-producing P. aeruginosa isolates in humans. 

https://www.mdpi.com/2079-6382/10/5/548