摘要

       在本文中，我们旨在使用下一代测序 (NGS) 来表征感染多重耐药大肠杆菌的健康和患病受试者的粪便微生物组及其耐药组。 在初步筛选后，选择了属于健康（n = 13）和患病（n = 13）受试者的 26 份粪便样本并进行 NGS。 在健康和患病受试者的耐药组中，分别鉴定出 23 和 42 个抗生素耐药基因 (ARG)，分别对 6 类和 9 类抗生素具有耐药性。 发现拟杆菌是健康和患病受试者的主要门； 然而，变形菌主要仅存在于患病受试者中。 微生物失调和患病受试者抵抗组中各种 ARG 的优势反映了巴基斯坦过度使用抗生素，需要立即关注以规范各种抗菌剂的使用。

       In this paper, we aimed to characterize the fecal microbiome and its resistomes of healthy and diseased subjects infected with multidrug-resistant Escherichia coli using next-generation sequencing (NGS). After initial screening, 26 stools samples belonging to healthy (n = 13) and diseased subjects (n = 13) were selected and subjected to NGS. A total of 23 and 42 antibiotic-resistant genes (ARGs) conferring resistance to 6 and 9 classes of antibiotics were identified in the resistomes of healthy and diseased subjects, respectively. Bacteroidetes were found to be the major phylum in both healthy and diseased subjects; however, Proteobacteria was predominantly present in the diseased subjects only. Microbial dysbiosis and predominance of various ARGs in the resistome of diseased subjects reflect the excessive usage of antibiotics in Pakistan and warrants immediate attention to regulate the use of various antimicrobials. View Full-Text

https://www.mdpi.com/2076-2607/9/3/616