摘要

       2017 年 7 月至 8 月，在中国 31 个省级行政区的 71 个城市（包括 28 个省会城市或直辖市、20 个地级市和 23 个县）采集了饮用水样本。此外，24 个抗生素抗性基因（ARGs）、通过qPCR对16S rRNA和2个整合酶基因进行定量，以研究ARGs的污染程度。结果显示，饮用水中的16S浓度为105-108拷贝/100mL，其处理过程可有效去除细菌。此外，磺胺类-ARGs是中国饮用水中最普遍的ARGs，且blaTEM在所选ARGs中所有城市的丰度排名前五，表明该基因的污染状况应引起更多关注。 qPCR 和相关性分析的数据表明 intI1 在饮用水中 ARGs 的传播中比 intI2 发挥更关键的作用。此外，不同城市类型间ARGs的污染程度无显着差异。

       Drinking water samples were collected from 71 cities, including 28 provincial capital cities or municipalities, 20 prefecture cities and 23 counties, of 31 provincial-level administrative regions in China from July to August in 2017. Futhermore, 24 Antibiotic Resistance Genes (ARGs), 16S rRNA and 2 integrase genes were quantified by qPCR to investigate the pollution degree of ARGs. The results revealed that the 16S ranged from 105 – 108 copies/100 mL in the drinking water, and its treatment process could effectively remove bacteria. Moreover, sulfonamides-ARGs were the most prevalent ARGs in the drinking water of China, and the abundance of blaTEM ranked top five in all cities among the selected ARGs, indicating that the pollution condition of the genes should be aroused more attention. The data of qPCR and correlation analyses indicated that intI1 played a more crucial role than intI2 in the propagation of ARGs in the drinking water. Additionally, the pollution degree of ARGs among different city types showed no significant difference.

https://www.sciencedirect.com/science/article/abs/pii/S0147651319311686