摘要

       抗生素抗性基因（ARGs）的广泛传播引起了越来越多的关注。然而，空气传播颗粒中 ARG 的发生和多样性仍有待了解。在这项研究中，大气中的总悬浮颗粒 (TSP) 来自典型的 ARG 污染源，包括动物养殖场和污水处理厂 (WWTP)，以及中国珠海市中心地区。宏基因组分析表明，来自动物农场和污水处理厂的 TSP 中的 ARG 丰富多样，但观察到这些样品之间 ARG 组成模式存在显着差异。与氨基糖苷类、大环内酯-林可酰胺-链霉素（MLS）和四环素抗性相关的 ARGs 在动物养殖场的 TSP 中比其他 ARGs 占优势，而多药和杆菌肽抗性基因在 WWTP 的 TSP 中比其他 ARGs 更丰富。在动物养殖场中，TSP 的 ARG 谱与动物粪便的谱图一致，表明动物粪便可能是动物养殖场中空气中 ARG 的主要来源之一。与 ARG 污染的代表性来源相比，从市区收集的 TSP 中 ARG 的丰度和多样性相对较低，多药耐药基因占主导地位。这项研究表明，对空气中 TSP 中 ARG 的宏基因组分析可以增强我们对 ARG 在环境中传播及其潜在健康威胁的全面了解。

        The wide spread of antibiotic resistance genes (ARGs) has attracted increasing concern. However, the occurrence and diversity of ARGs in airborne particles remains to be understood. In this study, total suspended particles (TSP) in the atmosphere were collected from typical sources of ARG pollution, including animal farms and wastewater treatment plant (WWTP), as well as the downtown area in Zhuhai, China. Metagenomic profiling demonstrated that ARGs were abundant and diverse in the TSP from animal farms and WWTP, but significant differences in ARG composition pattern between these samples were observed. ARGs associated with the resistance to aminoglycoside, macrolide-lincosamide-streptogramin (MLS) and tetracycline were dominant over other ARGs in the TSP of the animal farms, whereas multidrug and bacitracin resistance genes were more abundant than other ARGs in the TSP of the WWTP. In the animal farms, ARG profiles of the TSP were consistent with those of animal feces, indicating that animal feces could be one of the most contributing sources of airborne ARGs in animal farms. In contrast to representative sources of ARG pollution, ARG abundance and diversity in the TSP collected from the downtown area was relatively low, with multidrug resistance genes being predominant. This study suggests that metagenomic profiling of the ARGs in airborne TSP could enhance our comprehensive understanding of ARGs dissemination in the environment and their potential health threats.

https://www.sciencedirect.com/science/article/abs/pii/S0045653518317211