摘要

       猪粪是抗生素抗性基因 (ARG) 的重要储存库，但厌氧共消化 (AcoD) 可能会降低这些 ARG 的丰度。 然而，很少有研究考虑中草药残留（CMHRs）对 AcoD 期间 ARGs 和移动遗传元件（MGEs）变化的影响。 因此，本研究探讨了 CMHR 和猪粪 AcoD 期间 ARG 和 MGE 的命运。 结果表明，CMHRs 有效地降低了 AcoD 后主要 ARGs（不包括 ermF、qnrA 和 tetW）和四种 MGEs 的丰度（36.7-96.5%）。 冗余分析表明，细菌群落的变化主要影响 ARGs 的命运，而不是 MGEs 的水平基因转移。 网络分析表明，17 个细菌属可能是 ARGs 的宿主。 这项研究的结果表明，AcoD 与 CMHR 可用于从猪粪中去除一些 ARG 和 MGE。 

       Swine manure is an important reservoir for antibiotic resistance genes (ARGs) but anaerobic co-digestion (AcoD) can potentially reduce the abundance of these ARGs. However, few studies have considered the effects of Chinese medicinal herbal residues (CMHRs) on the variations in ARGs and mobile genetic elements (MGEs) during AcoD. Thus, this study explored the fate of ARGs and MGEs during the AcoD of CMHRs and swine manure. The results showed that CMHRs effectively reduced the abundances of the main ARGs (excluding ermF, qnrA, and tetW) and four MGEs (by 36.7–96.5%) after AcoD. Redundancy analysis showed that changes in the bacterial community mainly affected the fate of ARGs rather than horizontal gene transfer by MGEs. Network analysis indicated that 17 bacterial genera were possible hosts of ARGs. The results of this study suggest that AcoD with CMHRs could be employed to remove some ARGs and MGEs from swine manure.

https://www.sciencedirect.com/science/article/abs/pii/S0960852417319405