摘要

       水源性抗生素抗性基因 (ARG) 的广泛传播对数百万人的健康构成了日益严重的威胁。我们的研究检测了上海河网中 10 个 ARG 亚型的相对丰度，其中主要的 ARG 成分是 strB、sul1 和 ermB。这些 ARGs 被分别到达苏州 (SZ)、滇浦 (DP) 和黄浦 (HP) 河的污水、城市污水处理厂尾水和农业区径流显着富集（单向方差分析， P<0.01）。目标 ARG 以不同的模式分布在不同的河流中。 blaCTX-M和blaTEM有助于受城市源影响的河流中总ARGs的增加，特别是在深圳河中，其ARGs的分布与整个河网的汇合处显着相关（Mantel检验，P<0.01 ）。细菌群落与 ARG 结构紧密，潜在病原菌与目标 ARG 的关联在下游样品中变得显着（Procrustes 检验，P=0.03）。观察到城市污水沉降附近的水在DP河中具有最高的intl1含量，其下游样本的intl-ARG关系符合与网络汇合处相同的回归模型（R = 0.84，P <0.001）。河流水质的改善不会减少 ARGs，但可能会影响它们在上海河网中的分布格局。

       The widespread of water borne antibiotic resistance genes (ARGs) represents a growing threat to the health of millions of people. Our study detected the relative abundances of 10 ARG subtypes in the Shanghai river network, where the major ARG components were strB, sul1, and ermB. These ARGs were significantly enriched by the combined sewage, tail water from urban wastewater treatment plant and runoff from agricultural areas, which reached the Suzhou (SZ), Dianpu (DP), and Huangpu (HP) River, respectively (one-way ANOVA, P < 0.01). The target ARGs were distributed in varying patterns across different rivers. blaCTX-M and blaTEM contributed to the increase of total ARGs in the rivers influenced by urban sources, particularly in the SZ River, whose distribution of ARGs was significantly related to that of the confluence of the whole river network (Mantel test, P < 0.01). The bacterial community was closely structured with ARGs and potential pathogenic bacteria's association with target ARGs became significant in downstream samples (Procrustes test, P = 0.03). Water near urban wastewater fallouts was observed to have the highest content of intl1 in the DP River, whose downstream samples' intl –ARG relationship fitted the same regression model as that of the network confluence (R = 0.84, P < 0.001). The amelioration of river water quality does not reduce ARGs, but may affect their distributional patterns in the river network in Shanghai.

https://www.sciencedirect.com/science/article/abs/pii/S0043135419302234