摘要

       许多研究调查了多种环境中抗生素抗性基因 (ARG) 的组成和多样性，但对野外采集的土壤动物群中 ARG 的模式仍知之甚少。在本研究中，土壤跳虫从三个不同土地利用类型（公园路用地、公园用地和耕地）的六个地点收集，并对这些“野生”跳虫的微生物组中的 285 个 ARG 和 10 个移动遗传元件 (MGE) 进行了量化通过高通量定量 PCR。总共检测到 76 个独特的 ARG 和 5 个 MGE。跳虫中抗生素抗性组的收集位点之间存在显着差异。土地利用显着改变了弹尾藻 ARG 的分布模式。确定了 30 个共享的 ARG 和三个共享的 MGE。共享抗性组的共同出现在很大程度上是随机的，并且在协同网络中发现了更多的积极关系。部分冗余分析证实，细菌群落的变化解释了 ARG 变异的 27.77%。这些发现表明，抗性基因在与田间跳虫相关的微生物组中普遍存在，并且跳虫的活性可能有助于抗性基因在环境中的传播和传播，这是 ARGs 的一个方面，直到现在在很大程度上被忽视了。

       Numerous studies have investigated the composition and diversity of antibiotic resistance genes (ARGs) in multiple environments but the pattern of ARGs in field-collected soil fauna remains poorly understood. In the present study soil collembolans were collected from six sites with three different land use types (parkway land, park land, and arable land) and 285 ARGs and 10 mobile genetic elements (MGEs) in the microbiome of these “wild” collembolans were quantified by high-throughput quantitative PCR. A total of 76 unique ARGs and 5 MGEs were detected. There were significant differences between collection sites in the antibiotic resistome in the collembolans. Land use significantly altered the distribution patterns of collembolan ARGs. Thirty shared ARGs and three shared MGEs were identified. The co-occurrences of shared resistomes were largely random, and more positive relationships were found in the coassociation network. Partial redundancy analysis confirms that the changes in bacterial communities explained 27.77% of the variation in ARGs. These findings suggest that resistance genes are pervasive in the microbiome associated with the field collembolan and the activity of the collembolans may contribute to the spread and dissemination of resistance genes in the environment, an aspect of ARGs that has until now been largely overlooked.

https://pubs.acs.org/doi/abs/10.1021/acs.est.8b05116