摘要

       建立了三种废弃活性污泥 (WAS) 厌氧消化 (AD) 工艺，包括对照 (mono-WAS)、一级厌氧消化和二级厌氧消化以及微波-H2O2 预处理 (MW-H2O2) 以研究通过 qPCR 和高通量测序方法从重金属、intI1 和微生物群落中共同选择抗生素抗性基因 (ARG) 分布的概况和驱动因素。结果表明，MW-H2O2 可以降低所有 ARGs 的绝对基因拷贝，同时增加大多数 ARGs 的相对丰度。在随后的 AD 之后，总 ARGs 数量和相对丰度都得到了丰富，而两阶段 AD 显示出优于 ARGs 丰度减少的一些优势。此外，AD 在减少潜在病原体方面比 MW-H2O2 更有效。 AD可以降低intI1对ARGs传播的作用，而mantel test和procrustes分析表明ARGs丰度的变化与细菌群落的差异密切相关。

       Three anaerobic digestion (AD) processes of waste activated sludge (WAS) were established including the control (mono-WAS), one-stage AD and two-stage AD along with microwave-H2O2 pre-treatment (MW-H2O2) to investigate the profiles and drivers of antibiotic resistance genes (ARGs) distribution concerning co-selection from heavy metals, intI1 and microbial community through qPCR and high-throughput sequencing method. Results showed that MW-H2O2 could reduce the absolute gene copies of all ARGs while increased the relative abundance of most ARGs. After subsequent AD, both total ARGs quantities and relative abundance were enriched while two-stage AD showed some advantages over ARGs abundance reduction. Besides, AD was more effective on the potential pathogens reduction than MW-H2O2. AD could reduce the role of intI1 on the spread of ARGs, while mantel test and procrustes analysis indicated that the variation of ARGs abundance was closely associated with the discrepancy of bacterial community.

https://www.sciencedirect.com/science/article/abs/pii/S0960852417308258