摘要

       抗生素抗性基因 (ARGs) 被认为是城市污水处理厂 (WWTP) 废水中的一种新兴污染物，因为它们在重新用于景观和灌溉时对人类健康和生态安全具有潜在风险。传统的废水处理工艺通常无法有效减少 ARGs，尤其是细胞外 ARGs（eARGs），它们在环境中持久存在并通过转化在水平基因转移中发挥重要作用。在此，开发了一种预混和微滤的集成工艺，用于从废水中去除 ARG，尤其是 eARG。结果表明，集成过程可以有效降低流出物中总 ARGs (tARGs) (>2.9 log) 和 eARGs (>5.2 log) 的绝对丰度。优异的性能主要归功于在随后的微滤过程中通过预凝结和共排斥来捕获抗生素抗性细菌 (ARB) 和 eARG。此外，该集成工艺在去除废水中常见的污染物（例如溶解的有机碳和磷酸盐）以改善水质方面表现出良好的性能。此外，与微滤相比，集成工艺还大大减少了膜污染。这些发现表明，预混凝和微滤的综合过程是一种很有前景的先进废水处理技术，可用于从污水处理厂出水中去除 ARGs（尤其是 eARGs），以确保水回用安全。

       Antibiotic resistance genes (ARGs) have been regarded as an emerging pollutant in municipal wastewater treatment plant (WWTP) effluents due to their potential risk to human health and ecological safety when reused for landscape and irrigation. Conventional wastewater treatment processes generally fail to effectively reduce ARGs, especially extracellular ARGs (eARGs), which are persistent in the environment and play an important role in horizontal gene transfer via transformation. Herein, an integrated process of pre-coagulation and microfiltration was developed for removal of ARGs, especially eARGs, from wastewater effluent. Results show that the integrated process could effectively reduce the absolute abundances of total ARGs (tARGs) (>2.9 logs) and eARGs (>5.2 logs) from the effluent. The excellent performance could be mainly attributed to the capture of antibiotic resistant bacteria (ARB) and eARGs by pre-coagulation and co-rejection during subsequent microfiltration. Moreover, the integrated process exhibited a good performance on removing common pollutants (e.g., dissolved organic carbon and phosphate) from the effluent to improve water quality. Besides, the integrated process also greatly reduced membrane fouling compared with microfiltration. These findings suggest that the integrated process of pre-coagulation and microfiltration is a promising advanced wastewater treatment technology for ARGs (especially eARGs) removal from WWTP effluents to ensure water reuse security.

https://www.sciencedirect.com/science/article/abs/pii/S0043135419303859