摘要

       全基因组测序的扩展和随后抗生素耐药性目标的增加为高通量 qPCR (HT-qPCR) 铺平了道路，可在单次运行中分析数百个抗菌素耐药基因 (ARG)。对 51 项选定研究进行了荟萃分析，以评估过去 7 年的 ARGs 丰度趋势。 WaferGenTM SmartChip 被发现是用于评估 ARG 的最广泛使用的 HT-qPCR 平台。到目前为止，已经在 HT-qPCR 上分析了来自世界不同地区的大约 1000 个环境样本（不包括生物复制品）。肠道微生物组研究中报告的计算检测频率和归一化 ARGs 丰度（ARGs/16S rRNA 基因）表明与其他环境基质相比，ARGs 呈低趋势。 HT-qPCR 数据分析中的差异给研究人员在结果的精确解释方面造成困难已经得到强调，并且还提出了解决这些问题的可能方法。审查中还讨论了其他放大技术和护理点或现场可部署设备用于分析 ARG 的潜力。我们的审查侧重于有关 HT-qPCR 在抗菌素耐药性领域的作用、现状和未来前景的最新信息。

       Expansion in whole genome sequencing and subsequent increase in antibiotic resistance targets have paved the way of high throughput qPCR (HT-qPCR) for analyzing hundreds of antimicrobial resistance genes (ARGs) in a single run. A meta-analysis of 51 selected studies is performed to evaluate ARGs abundance trends over the last 7 years. WaferGenTM SmartChip is found to be the most widely used HT-qPCR platform among others for evaluating ARGs. Up till now around 1000 environmental samples (excluding biological replicates) from different parts of the world have been analyzed on HT-qPCR. Calculated detection frequency and normalized ARGs abundance (ARGs/16S rRNA gene) reported in gut microbiome studies have shown a trend of low ARGs as compared to other environmental matrices. Disparities in the HT-qPCR data analysis which are causing difficulties to researchers in precise interpretation of results have been highlighted and a possible way forward for resolving them is also suggested. The potential of other amplification technologies and point of care or field deployable devices for analyzing ARGs have also been discussed in the review. Our review has focused on updated information regarding the role, current status and future perspectives of HT-qPCR in the field of antimicrobial resistance.

https://www.mdpi.com/1420-3049/24/1/163