摘要

       家畜粪便是抗生素抗性基因 (ARG) 的储存库。 在高固体厌氧消化 (HSAD) 过程中出现的内源性抑制剂极大地影响了生物过程性能和 ARG。 本研究调查了包括挥发性脂肪酸 (VFA) 和游离氨 (FA) 在内的内源性抑制剂对猪粪 HSAD 期间 ARG 发生的影响。 HSAD 期间的 ARG 特性（8%–14% 总固体（TS））与低 TS 控制（4%）有很大不同。 内源性抑制剂和微生物群落极大地促进了 ARGs 的三相变化。 VFAs 的浓度和 Proteobacteria 和 intI1 的丰度与 ARGs 的变化相关。 FA 抑制和 VFA 积累（尤其是丙酸盐）延迟并限制了 ARG 的下降。 最终产品中 ARG 的相对缓慢变化率和高 ARG 表明 HSAD 消化物用于土地应用的风险很高。

       Livestock manure is a reservoir of antibiotic resistance genes (ARGs). The endogenous inhibitors that emerge during high solid anaerobic digestion (HSAD) greatly influence the bioprocess performance and the ARGs. The effect of endogenous inhibitors including volatile fatty acids (VFAs) and free ammonia (FA) on the ARG occurrences during HSAD of swine manure was investigated in this study. The ARG properties during HSAD (8%–14% total solids (TS)) largely differed from the low TS control (4%). The endogenous inhibitors and microbial communities greatly contributed to the three-phase changes in ARGs. The concentrations of VFAs and abundances of Proteobacteria and intI1 were correlated with the changes in ARGs. FA inhibition and VFA accumulation (especially propionate) delayed and restricted the decline of ARGs. The relatively slow rate of changes in ARGs and high ARGs in the end product suggested the high risk of the HSAD digestate for land application.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418312963