摘要

       随着我国海水养殖的快速发展，大量抗生素被排放到水环境中。关于抗生素和与中国海水养殖环境相关的相应抗生素抗性基因 (ARG) 的信息很少。收集了中国整个海岸线 11 个典型海水养殖区的沉积物，并将中国南方梅吉角的沉积物用作非海水养殖控制。结果表明，抗生素及其相应的 ARGs 广泛分布于大多数海水养殖沉积物中，并且在美济礁样品中以低浓度存在。磺胺类耐药基因普遍存在，其中蓬莱地区sul1和sul2基因定量PCR检测最高。此外，观察到不同位点之间 ARG 的显着差异。由于检测ARGs的引物有限，illumina高通量测序也被用于分析ARGs，结果显示所有样品中bacA的丰度与其他ARGs相比最高，其次是mexF和mexB。这是第一项全面调查中国沿海典型海水养殖区抗生素耐药性的研究。这项研究提供了对海水养殖对中国细菌和 ARG 组成谱影响的见解。

       With the rapid development of mariculture in China, large amounts of antibiotics are being discharged into the aquatic environment. Little information is available regarding antibiotics and corresponding antibiotic resistance genes (ARGs) associated with maricultural environments in China. Sediments from eleven typical mariculture areas along the whole coastline of China were collected, and the sediment in Meijijiao in southern China was used as a non-mariculture control. The results revealed that antibiotics and their corresponding ARGs were widely distributed in most maricultural sediments, and present at low concentrations in samples from Meijijiao. The sulfonamide-resistance genes were prevalent, and the sul1 and sul2 in Penglai were the highest detected by using quantitative PCR. Moreover, remarkable differences in ARGs among different sites were observed. Due to the limited availability of primers to detect ARGs, illumina high-throughput sequencing was also used for profiling ARGs, and the results showed that the abundance of bacA in all samples was the highest compared to other ARGs, followed by mexF and mexB. This is the first study to comprehensively investigate the antibiotic resistance profile in typical mariculture areas along the whole coast of China. This study provides insights into the impacts of mariculture on the profiles of bacterial and ARG compositions in China.

https://www.sciencedirect.com/science/article/abs/pii/S0048969718305126