摘要

       抗生素抗性基因（ARGs）对各种环境的污染是一个亟待解决的问题，尤其是在重金属污染的环境中。 本研究调查了 ARG 和移动遗传元件 (MGE) 对在厌氧消化过程中向含有高浓度铜的猪粪添加氧化石墨烯 (GO) 的反应。 由于铜的压力，ARGs 和 MGEs 的总拷贝数显着增加。 GO 显着降低了 ARG 和 MGE 拷贝数，其中低 GO 浓度比高 GO 浓度表现更好。 网络分析表明，大多数 ARG 和 MGE 共同发生，并且它们共享相同的主要潜在宿主细菌。 通过冗余分析评估了不同因素对 ARG 丰度的贡献，并且 MGE 对 ARG 的命运有最重要的影响。 因此，GO 可能主要通过去除 MGE 来减少 ARG 的丰度。 

       The pollution of various environments with antibiotic resistance genes (ARGs) is an urgent problem that needs to be addressed, especially in heavy metal-polluted environments. This study investigated the responses of ARGs and mobile genetic elements (MGEs) to the addition of graphene oxide (GO) to swine manure containing a high concentration copper during anaerobic digestion. The total copy numbers of ARGs and MGEs were significantly enhanced by the pressure due to Cu. GO significantly decreased the ARG and MGE copy numbers, where the low GO concentration performed better than the high GO concentration. Network analysis showed that most of the ARGs and MGEs co-occurred and they shared the same major potential host bacteria. The contributions of different factors to ARG abundances were assessed by redundancy analysis and MGEs had the most important effect on the fate of ARGs. Thus, GO may reduce the abundance of ARGs mainly by removing MGEs.

https://www.sciencedirect.com/science/article/abs/pii/S0048969718344462