摘要

使用有机肥增加了抗生素抗性基因（ARG）的环境负担。我们调查了四环素（tet）抗性基因和细菌类群对两月内用四环素修饰的粪肥应用的反应。对代表性四环素（土霉素，氯四环素和多西环素），tet抗性基因（tet（M），tet（O），tet（W），tet（S），tet（Q）和tet（X））和未处理土壤，+粪肥组和+粪肥+四环素组进行了分析。在第1天，+粪肥组中所有tet抗性基因的丰度显着高于未处理的土壤组。在第30天和第60天时，除tet（Q）和tet（X）外，+粪肥组所有tet抗性基因的丰度显着低于比+粪+四环素组。+粪肥组的耗散率高于+粪肥和+四环素组。还观察到由四环素对土壤细菌群落组成的干扰。结果表明，施用四环素可减缓施肥后耕地土壤中tet抗性基因的耗散。施用经四环素修饰的粪肥可为小单孢菌科，丙酸杆菌科，链霉菌科，亚硝化螺菌科和梭菌科等分类科的物种提供显着的选择优势。

Manure application contributes to the increased environmental burden of antibiotic resistance genes (ARGs). We investigated the response of tetracycline (tet) resistance genes and bacterial taxa to manure application amended with tetracyclines over two months. Representative tetracyclines (oxytetracycline, chlorotetracycline and doxycycline), tet resistance genes (tet(M), tet(O), tet(W),tet(S), tet(Q) and tet(X)) and bacterial taxa in the untreated soil, +manure, and +manure+tetracyclines groups were analyzed. The abundances of all tet resistance genes in the +manure group were significantly higher than those in the untreated soil group on day 1. The abundances of all tet resistance genes (except tet(Q) andtet(X)) were significantly lower in the +manure group than those in the +manure+tetracyclines group on day 30 and 60. The dissipation rates were higher in the +manure group than those in the +manure+tetracyclines group. Disturbance of soil bacterial community composition imposed by tetracyclines was also observed. The results indicated that tetracyclines slowed down the dissipation of tetresistance genes in arable soil after manure application. Application of manure amended with tetracyclines may provide a significant selective advantage for species affiliated to the taxonomical families of Micromonosporaceae,Propionibacteriaceae, Streptomycetaceae, Nitrospiraceae and Clostridiaceae.

https://www.sciencedirect.com/science/article/pii/S0147651317305596