摘要

       堆肥通常用于处理牲畜粪便，接种木质纤维素降解微生物可以提高堆肥质量。在本研究中，在猪粪与废弃蘑菇基质共堆肥过程中，以两个水平接种木质纤维素降解微生物（未接种对照=0%，T 处理=10%），及其对抗生素抗性基因（ARGs）和对细菌群落进行了调查。与对照相比，接种木质纤维素降解微生物导致 6/11 ARGs 和 3/4 移动遗传元件的降低更大。共堆肥后，ARGs 的总相对丰度在对照中增加了 0.23 个对数，但在 T 处理中减少了 0.08 个对数。两种处理中细菌群落根据堆肥时间进行聚类，其中接种主要影响嗜温阶段的细菌群落。冗余分析和网络分析表明细菌群落演替对ARGs的变异有重要影响。接种木质纤维素降解微生物导致 ARG 减少，这与 ARG 潜在宿主细菌的丰度显着相关。因此，接种木质纤维素降解微生物可以降低 ARG 传播的风险，并使堆肥产品更加安全。

       Composting is usually employed to treat livestock manure, and inoculation with lignocellulose-degrading microorganisms can enhance the quality of compost. In this study, lignocellulose-degrading microorganisms were inoculated at two levels (uninoculated control = 0%, and T treatment = 10%) during co-composting of swine manure with spent mushroom substrate, and their effects on antibiotic resistance genes (ARGs) and the bacterial community were investigated. Inoculation with lignocellulose-degrading microorganisms caused greater decreases in 6/11 ARGs and 3/4 mobile genetic elements than the control. The total relative abundances of ARGs increased by 0.23 logs in the control but decreased by 0.08 logs in the T treatment after co-composting. The bacterial community was clustered according to the composting time in the two treatments, where inoculation mainly affected the bacterial community during the mesophilic phase. Redundancy analysis and network analysis showed that the bacterial community succession had important effects on the variations in ARGs. Inoculation with lignocellulose-degrading microorganisms led to the reduction of ARGs, which was significantly correlated with the abundances of potential host bacteria for ARGs. Thus, inoculation with lignocellulose-degrading microorganisms could decrease the risk of ARGs spreading and make compost products more security.

https://www.sciencedirect.com/science/article/abs/pii/S0269749118358342