摘要

       环境中的抗生素抗性基因（ARGs）因其健康风险而备受关注。我们调查了中国流溪河沉积物和水样中的抗生素浓度（四环素类、磺胺类和氟喹诺酮类）、ARGs 丰度（四环素、磺胺类和质粒介导的喹诺酮类抗性 (PMQR) 基因）和细菌群落组成。通过超高效液相色谱-电喷雾串联质谱法测定抗生素浓度。 ARGs 丰度通过独立于培养的方法进行量化。基于Ion Torrent Personal Genome Machine平台，通过宏基因组方法分析细菌群落组成。沉积物样品中抗生素浓度为 1.19–622 ug kg-1，低于水样中 127 ng L-1 的检测限。检测到的 ARGs 的相对丰度（ARGs 拷贝/16S rRNA 基因拷贝）在 10-5 到 10-2 的范围内。沉积物样品中的优势门为变形菌门、拟杆菌门和疣微菌门，水样中的优势门为变形菌门、放线菌门和拟杆菌门。结果表明，河流环境受到抗生素污染，可能是 ARGs 的蓄水池。本研究提供了流溪河中抗生素、ARGs 和细菌群落组成的定量数据，流溪河的地理位置与报道的研究不同。

       Antibiotic resistance genes (ARGs) in the environment have paid great concern due to their health risk. We investigated antibiotics concentrations (tetracyclines, sulfonamides, and fluoroquinolones), ARGs abundances (tetracycline, sulfonamide, and plasmid-mediated quinolone resistance (PMQR) genes), and bacterial community composition in sediment and water samples in the Liuxi River, China. Antibiotics concentrations were determined by ultra-performance liquid chromatography-electrospray tandem mass spectrometry. ARGs abundances were quantified by a culture-independent method. Bacterial community composition was analyzed by metagenomic approach based on Ion Torrent Personal Genome Machine platform. Antibiotics concentrations were at the levels of 1.19–622 ug kg−1 in sediment samples and below the limit of detection to 127 ng L−1 in water samples. Relative abundances (ARGs copies/16S rRNA gene copies) of detected ARGs were at the range of 10−5 to 10−2. The dominant phyla were Proteobacteria, Bacteroidetes, and Verrucomicrobia in sediment samples, and were Proteobacteria, Actinobacteria, and Bacteroidetes in water samples. The results indicated that the river environment was contaminated by antibiotics and may be as a reservoir of ARGs. This study provided quantitative data on antibiotics, ARGs and bacterial community composition in the Liuxi River, a geographical location different from the reported studies.

https://www.frontiersin.org/articles/10.3389/fenvs.2014.00061/full