摘要

       堆肥在减少动物粪便中的抗生素抗性基因 (ARG) 方面并不完全有效。这项工作研究了不同处理条件对以花椰菜和玉米秸秆为填充剂的猪粪堆肥中 ARGs 归宿的影响。结果表明，与对照处理相比，微生物制剂的添加或玉米秸秆与花椰菜的比例（1:12）可显着降低大多数ARGs的绝对丰度（平均480倍）。主成分分析表明，细菌群落与 ARG 丰度显着相关，表明微生物群落对共堆肥过程中的 ARG 变异有影响。冗余和网络分析证实，个体 ARG（qnrS、blaAmpC、blaTEM-1）的变化模式受宿主细菌（假单胞菌、克雷伯氏菌和盐球藻）和环境变量（TN、NH3-N、TOC 和 pH 值）的选择性影响）。这些发现有助于优化堆肥条件，从而降低 ARG 传播的风险。

       Composting is not completely effective in reducing antibiotic resistance genes (ARGs) in animal manure. This work studied the effects of different treatment conditions on the fate of ARGs in composting swine manure with cauliflower and corn straw as bulking agents. The results showed that the addition of microbial agents or the ratio of corn stalks to cauliflower (1:12) could significantly decrease the absolute abundances of most ARGs (an average of 480 times) compared with the control treatment. Principal component analysis indicated that bacterial communities were significantly correlated with ARG abundance, suggesting that microbial communities have an impact on ARG variation during co-composting. Redundancy and Network analysis confirmed the changing patterns of individual ARGs (qnrS, blaAmpC, blaTEM-1) were influenced by the selectivity of host bacteria (Pseudomonas, Klebsiella, and Halocella) and environmental variables (TN, NH3-N, TOC, and pH). These findings helped to optimize composting conditions, thereby reducing the risk of ARGs spread.

https://www.sciencedirect.com/science/article/abs/pii/S096085241931898X