摘要

       多年来，废水污泥中的抗生素污染和相关的抗生素抗性基因 (ARG) 一直是环境污染和人类健康风险的一个问题。本研究调查了来自污泥处理湿地 (STW) 的污泥中抗生素和相关 ARG 的归宿。我们检查了三个污泥处理床，即一号机组是带曝气管的污泥干燥床； 2号机组为通风污泥干化芦苇床； 3号机组为无曝气管的污泥干化芦苇床。靶向抗生素包括土霉素、罗红霉素和阿奇霉素。靶向 ARG 包括 tetA、tetC、msrSA 和 ermB。结果表明，在所有三个单位中，抗生素都被显着去除，并且相关的 ARG 在一年内下降。表层抗生素浓度低于底层。在 2 号单元中观察到目标抗生素的最高去除效率。目标 ARG 的去除效率受不同参数的影响，尤其是簧片、曝气管和温度。抗生素浓度与相应ARGs的相关性分析表明，在所有三个单元的底层和表层，罗红霉素和阿奇霉素的浓度与msrSA的绝对丰度之间均存在显着相关性（p<0.05）。在土霉素浓度与 tetA 和 tetC 的绝对丰度之间也观察到显着相关性 (p<0.05)。结果表明，STWs能有效降低目标抗生素含量及相关ARGs；带有簧片和曝气管的 STW 表现更好。

       Antibiotics contamination and related antibiotics resistance genes (ARGs) in wastewater sludge have been a concern for environmental pollution and human health risk for years. This study investigated the fate of antibiotics and related ARGs in sludge from sludge treatment wetlands (STWs). We examined three sludge treatment beds i.e. unit No.1 was sludge drying bed with aeration tubes; unit No.2 was a ventilated sludge drying reed bed; and the unit No.3 was a sludge drying reed bed without aeration tubes. The targeted antibiotics included oxytetracycline, roxithromycin and azithromycin. The targeted ARGs included tetA, tetC, msrSA and ermB. The results indicated that in all three units antibiotics were removed significantly and related ARGs declined over one year period. The antibiotics concentrations in the surface layer were lower than those in the bottom layer. The highest removal efficiency of the targeted antibiotics was observed in the unit No.2. The removal efficiency of the targeted ARGs influenced by different parameters, especially reeds, aeration tubes and temperature. Correlation analysis between the concentrations of antibiotics and corresponding ARGs showed that in both the bottom and surface layer of all three units, a significant correlation (p < 0.05) was found between the concentrations of roxithromycin and azithromycin and the absolute abundances of msrSA. A significant correlation (p < 0.05) was also observed between the concentrations of oxytetracycline and the absolute abundances of tetA and tetC. The results demonstrated that STWs can effectively reduce the target antibiotics contents and related ARGs; and the STW with reed and aeration tubes performed better.

https://www.sciencedirect.com/science/article/abs/pii/S0045653519303923