摘要

在这项研究中，在中试规模中研究了一种用于猪废水处理，缺氧和四阶段微氧曝气序列以及凝结过程（AO4）的新工艺，以确定其去除抗生素和抗生素抗性基因（ARG ）。结果表明，长期低溶解氧（DO）运行条件对降低ARGs的抗生素和相对丰度起着重要作用。在AO4过程中，大约98％的抗生素被除去，同时ARGs拷贝数减少了4-6个数量级。低DO（0.15mg / L和0.10mg / L）的AO4工艺中细菌内源衰减率的降低可能导致细菌总体大小和污泥浓度的增加。结果，高生物量加速了污泥中抗生素的积累。此外，实时聚合酶链式反应扩增（qPCR）分析表明，长期低DO可以通过富集活性污泥中的16S rDNA并抑制ARGs的出现或分布来降低ARG的相对丰度。我们的研究结果意味着AO4工艺不仅可以显着减少常规污染物，如化学需氧量（COD），化学需氧量（COD），总氮（TN）和总磷（TP），还可以显着减少抗生素和ARGs。

In this study, a novel process for swine wastewater treatment, anoxic and four-stage micro oxygen aerated sequence coupled with a coagulation process (AO4) was investigated in the pilot scale to determine its capacity in the removal of antibiotics and antibiotic resistance genes (ARGs). Results showed that long-term low dissolved oxygen (DO) operational condition played a significant role in decreasing the antibiotics and relative abundance of ARGs. Around 98% of the total antibiotics were removed along with 4–6 orders magnitude reductions of the ARGs copy numbers in AO4 process. Reduction of bacterial endogenous decay rate in AO4 process with low DO (0.15 mg/L and 0.10 mg/L) might result in the increase in the total bacterial population size and the concentration of sludge. As a result, high biomass accelerated the accumulation of antibiotics in sludge. In addition, real-time polymerase chain reaction amplification (qPCR) assays indicated that the long-term low DO could reduce the relative abundance of ARGs via enriching the 16S rDNA in activated sludge and inhibiting the emergence or distribution of ARGs. Our findings imply that the AO4 process could not only achieve a significant reduction of conventional contaminants such as chemical oxygen demand (COD), chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) but also antibiotics and ARGs.

https://www.sciencedirect.com/science/article/pii/S1385894717317576