摘要

       抗生素抗性细菌 (ARB) 和抗生素抗性基因 (ARG) 在各种环境基质中的普遍存在是对人类和生态健康的潜在威胁。因此，使用光电催化 (PEC) 过程研究了 ARB 大肠杆菌 S1-23 的灭活及其相关 ARG、blaTEM-1 和 aac(3)-II 的消除。结果表明，ARB 大肠杆菌 S1-23（1 × 108 cfu mL-1）及其 ARG（细胞外和细胞内）可以分别在 PEC 处理 10 和 16 小时内完全灭活。相比之下，光催化（PC）和电化学（EC）处理没有显示出明显的效果；然而，从大肠杆菌 S1-23 中提取的含有 ARG 的 DNA，用作解离裸 ARG 的模型，可以通过这三种处理在几分钟内完全分解。进一步的分析，包括 PCR、AFM 和 HPLC，证明裸 ARG 的结构完整性和表面形貌在治疗过程中受到破坏，可以完全消除。此外，在 PEC、PC 和 EC 处理过程中不会产生胞嘧啶、鸟嘌呤、腺嘌呤或胸腺嘧啶中间体。这项研究是首次提出将 PEC 处理作为完全分解水系统中 ARB 和 ARG 的有前途的方法的报告。

       The ubiquity of antibiotic-resistance bacteria (ARB) and antibiotic-resistance genes (ARGs) in various environmental matrices is a potential threat to human and ecological health. Therefore, the inactivation of ARB E. coli S1-23 and the elimination of its associated ARGs, blaTEM-1 and aac(3)-II, were investigated using the photoelectrocatalytic (PEC) process. Results indicate that the ARB E. coli S1-23 (1 × 108 cfu mL−1) and its ARGs (extracellular and intracellular) could be fully inactivated within 10 and 16 h PEC treatment, respectively. In contrast, photocatalytic (PC) and electrochemical (EC) treatments displayed no obvious effect; however, ARG-containing DNA extracted from E. coli S1-23, which was used as a model for dissociative naked ARGs, could be completely decomposed within a few minutes through these three treatments. Further analyses, including PCR, AFM and HPLC, proved that the structural integrity and surface topography of naked ARGs are damaged during treatment and can be completely eliminated. Furthermore, there is no generation of cytosine, guanine, adenine or thymine intermediates during the PEC, PC, and EC treatments. This study is the first report to propose the PEC treatment as a promising method for complete decomposition of ARB and ARGs in aqueous systems.

https://www.sciencedirect.com/science/article/abs/pii/S0043135417307121