摘要

       在这项研究中，在中试规模中研究了一种新的猪废水处理工艺、缺氧和四阶段微氧曝气序列与混凝工艺 (AO4) 相结合，以确定其去除抗生素和抗生素抗性基因 (ARGs) 的能力。 ）。结果表明，长期低溶解氧 (DO) 操作条件在降低抗生素和 ARGs 的相对丰度方面发挥了重要作用。在 AO4 过程中，大约 98% 的抗生素被去除，同时 ARGs 拷贝数减少了 4-6 个数量级。降低 AO4 过程中低 DO（0.15 mg/L 和 0.10 mg/L）细菌内源性腐烂率可能会导致总细菌种群规模和污泥浓度的增加。结果，高生物量加速了污泥中抗生素的积累。此外，实时聚合酶链反应扩增（qPCR）分析表明，长期低溶解氧可以通过富集活性污泥中的 16S rDNA 并抑制 ARGs 的出现或分布来降低 ARGs 的相对丰度。我们的研究结果表明，AO4 工艺不仅可以显着减少传统污染物，如化学需氧量 (COD)、化学需氧量 (COD)、总氮 (TN) 和总磷 (TP)，还可以显着减少抗生素和 ARG。

       In this study, a novel process for swine wastewater treatment, anoxic and four-stage micro oxygen aerated sequence coupled with a coagulation process (AO4) was investigated in the pilot scale to determine its capacity in the removal of antibiotics and antibiotic resistance genes (ARGs). Results showed that long-term low dissolved oxygen (DO) operational condition played a significant role in decreasing the antibiotics and relative abundance of ARGs. Around 98% of the total antibiotics were removed along with 4–6 orders magnitude reductions of the ARGs copy numbers in AO4 process. Reduction of bacterial endogenous decay rate in AO4 process with low DO (0.15 mg/L and 0.10 mg/L) might result in the increase in the total bacterial population size and the concentration of sludge. As a result, high biomass accelerated the accumulation of antibiotics in sludge. In addition, real-time polymerase chain reaction amplification (qPCR) assays indicated that the long-term low DO could reduce the relative abundance of ARGs via enriching the 16S rDNA in activated sludge and inhibiting the emergence or distribution of ARGs. Our findings imply that the AO4 process could not only achieve a significant reduction of conventional contaminants such as chemical oxygen demand (COD), chemical oxygen demand (COD), total nitrogen (TN) and total phosphorus (TP) but also antibiotics and ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S1385894717317576