摘要

       应用嗜热好氧消化 (TAD) 以进一步减少厌氧消化 (AnD) 污泥中的 ARGs 和重金属抗性基因 (HMRGs) 以及 1 类整合子 (intI1)。 与 AnD 不同，TAD 后没有 ARGs、HMRGs 和 intI1 的富集。 intI1 和总 ARG 的残留基因分数（靶向 ARG 的总和）分别为 0.03 和 0.08。 两个动力学模型（Collins-Selleck 和一阶）描述了目标基因的衰减模式，揭示了在 TAD 期间 intI1 的快速去除。 在 TAD 之后，与厌氧消化污泥相比，人类细菌病原体 (HBP) 的相对丰度和 HBP 种类的数量分别下降至约 68% 和 64%。 因此，继 AnD 之后的 TAD 可能具有降低由污水污泥中的 ARG、HMRG、intI1 和 HBP 引起的生物风险的巨大潜力。 

       Thermophilic aerobic digestion (TAD) was applied to further reduce ARGs and heavy metal resistance genes (HMRGs) as well as class 1 integrons (intI1) in sludge from anaerobic digestion (AnD). Unlike after AnD, there was no enrichment of ARGs, HMRGs and intI1 after TAD. Residual gene fractions of intI1 and total ARGs (sum of targeted ARGs) were 0.03 and 0.08, respectively. Two kinetic models (Collins-Selleck and first-order) described the decay patterns of targeted genes, revealing rapid removal of intI1 during TAD. After TAD, the relative abundance of human bacterial pathogens (HBPs) and the numbers of HBPs species decreased to approximately 68% and 64% compared to anaerobically digested sludge, respectively. Thus, TAD, subsequent to AnD, may possess high potential for reducing biological risks resulting from ARGs, HMRGs, intI1 and HBPs in sewage sludge.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418315475