摘要

       本研究探讨了猪粪与小麦秸秆的不同质量比（3:7、5:5 和 7:3，分别是对照反应器 C1、C2 和 C3）对抗生素抗性基因变异的影响。 ARGs) 和厌氧消化 (AD) 过程中的微生物群落。 C1、C2 和 C3 的累积沼气产量分别为 1711、3857 和 3226 mL。 AD后，ARGs的总相对丰度在C3中减少了4.23logs，而在C1和C2中分别减少了1.03和1.37logs。 网络分析表明，Solibacillus、Enterococcus、Facklamia、Corynebacterium_1 和 Acinetobacter 属是 ermB、sul1 和 dfrA7 的潜在宿主。 冗余分析表明，细菌群落和环境因素在 ARGs 的变异中起重要作用。 因此，在重复使用经过 AD 处理的动物粪便之前，应考虑减少 ARG。 

       This study explored the effects of different mass ratios of swine manure relative to wheat straw (3:7, 5:5, and 7:3, i.e., control reactors C1, C2, and C3, respectively) on variations in antibiotic resistance genes (ARGs) and the microbial community during anaerobic digestion (AD). The cumulative biogas production volumes were 1711, 3857, and 3226 mL in C1, C2, and C3, respectively. After AD, the total relative abundance of ARGs decreased by 4.23 logs in C3, whereas the reductions were only 1.03 and 1.37 logs in C1 and C2, respectively. Network analysis showed that the genera Solibacillus, Enterococcus, Facklamia, Corynebacterium_1, and Acinetobacter were potential hosts of ermB, sul1, and dfrA7. Redundancy analysis showed that the bacterial communities and environmental factors played important roles in the variation in ARGs. Thus, reductions in ARGs should be considered before reusing animal manure treated by AD.

https://www.sciencedirect.com/science/article/abs/pii/S0960852417300913