摘要

       抗生素抗性基因 (ARG) 是威胁人类健康的新兴微生物危害。许多包括城市和农业活动以及水环境变化在内的因素可以显着地影响 ARG 的变化。 ARGs 发生及变异的调查本研究以九龙江为例。水样采集自 16 个地点，范围从九龙江河口上游支流，分低流期和高流期。十一通过实时 PCR（SYBR Green）方法对 ARG 家族进行测试和定量，并检测对 tetA、tetG、aacC1、strA、ermB、cmlA5、vanA、dfrA1、sulII、blaTEM1 和blaoxa1 基因。结果显示发现了 9 个 ARG 家族，然后是养猪业和城市活动，包括污水排放，可能是造成高水平ARGs 的浓度和相对丰度，以及盐度的增加可能会降低ARGs 的相对丰度。此外，一些 ARG 丰度与NH4+ 和 PO43 的浓度，特别是在高流量时期。人类活动是九龙江ARGs的重要来源，已成为安全威胁为附近城市提供饮用水。

       Antibiotic resistance genes (ARGs) are emerging microbial hazards threatening human health. Many factors,including urban and agricultural activities and water environment changes, can significantly impact the variations of ARGs. An investigation on the occurrence and variations of ARGs in the Jiulong River was done in this study. The water samples were collected from 16 sites ranging from the upper branch to the river mouth of the Jiulong River, in both low­ and high­flow periods. Eleven ARG families were tested and quantified by real­time PCR (SYBR Green) methods, and detection assays were conducted for tetA, tetG, aacC1, strA, ermB, cmlA5, vanA, dfrA1, sulII, blaTEM­1 and
blaoxa­1 genes. Results showed that nine ARG families were found, then the swine industry and urban activities, including sewage discharge, might be responsible for the high levels of concentrations and relative abundances of ARGs, and the increase of salinity might decrease the relative abundances of ARGs. In addition, some ARG abundances were significantly correlated to the concentrations of NH4 + and PO4­3, particularly in the high­flow period. The human activities were important sources for ARGs in the Jiulong River, which have already become a threat to the safety
of drinking water for the nearby cities.

http://jeb.co.in/journal_issues/201304_apr13_supp/paper_09.pdf