摘要

       多重耐药菌对人类健康造成了巨大的全球负担，这可能是由移民浪潮推动的：2015 年，476,649 名难民在德国申请庇护，主要是由于叙利亚危机。 在阿拉伯国家，多重耐药菌给医疗保健系统带来了重大问题。 目前，没有数据描述健康难民的抗生素耐药性。 在这里，我们评估了难民和德国对照中的微生物景观和抗生素抗性基因 (ARG) 的存在。 为了实现这一目标，我们对 500 名连续的难民进行了系统研究，这些难民主要来自叙利亚、伊拉克和阿富汗，以及 100 名德国控制者。 对粪便样本进行基于 PCR 的 42 种最相关 ARG 定量、基于 16S 核糖体 RNA 基因测序的微生物群分析，以及基于培养的多重耐药微生物验证

       Multidrug-resistant bacteria represent a substantial global burden for human health, potentially fuelled by migration waves: in 2015, 476,649 refugees applied for asylum in Germany mostly as a result of the Syrian crisis. In Arabic countries, multiresistant bacteria cause significant problems for healthcare systems. Currently, no data exist describing antibiotic resistances in healthy refugees. Here, we assess the microbial landscape and presence of antibiotic resistance genes (ARGs) in refugees and German controls. To achieve this, a systematic study was conducted in 500 consecutive refugees, mainly from Syria, Iraq, and Afghanistan and 100 German controls. Stool samples were subjected to PCR-based quantification of 42 most relevant ARGs, 16S ribosomal RNA gene sequencing-based microbiota analysis, and culture-based validation of multidrug-resistant microorganisms.