摘要

       受到粪便污染的环境是抗生素耐药性的储存库。最近的宏基因组观察表明，粪便污染指标 crAssphage 与环境中抗生素抗性基因 (ARG) 的出现相关。扩大 crAssphage 的效用来代表 ARG 的环境发生可能会促进被粪便污染的环境中的 ARG 管理。在这项研究中，我们在 30 天的采样周期内分析了接收合并下水道溢流的城市河流中 ARG 和 crAssphage 的一系列分子指标。采样流显示出高水平的 ARGs 和 crAssphage，在潮湿天气事件期间具有统计学上显着升高的水平。当所有都使用数字液滴聚合酶链反应 (PCR) 进行测量时，观察到的 crAssphage 和 ARG 分子检测之间的相关性很高。 crAssphage 的定量 PCR 和数字液滴 PCR 定量仅显示中等一致性，强调了检测技术在进行定量比较时的重要性。总的来说，这项研究证明了当采用“工具箱”方法进行粪便污染管理时，crAssphage 粪便指标与 ARG 发生相关的潜力。

       Environments that receive fecal pollution are reservoirs of antibiotic resistance. Recent metagenomic observations suggest that the fecal pollution indicator crAssphage correlates with the occurrence of antibiotic resistance genes (ARGs) in the environment. Expanding the utility of crAssphage to represent the environmental occurrence of ARGs would potentially facilitate ARG management in environments contaminated with fecal pollution. In this study, we analyzed a suite of molecular indicators for ARGs and crAssphage over a 30 day sampling period in an urban stream that receives combined sewer overflows. The sampled stream showed high levels of ARGs and crAssphage with statistically significantly elevated levels during wet weather events. The observed correlation between crAssphage and ARG molecular detection was high when all were measured using digital droplet polymerase chain reaction (PCR). Quantitative PCR and digital droplet PCR quantifications of crAssphage showed only moderate agreement, emphasizing the importance of detection technology when making quantitative comparisons. Overall, this study demonstrates the potential of a crAssphage fecal indicator to correlate with ARG occurrence when employing a “toolbox” approach to fecal pollution management.
https://pubs.acs.org/doi/abs/10.1021/acs.estlett.9b00130