摘要

日益增加的耐药性感染引起了研究人员对环境细菌检测的研究兴趣，并且发现许多因素，包括金属条件的提高，都有导致抗生素抗性( AR )的扩散。本研究通过比较12种金属和13种与四环素、β-内酰胺和磺酰胺抗性相关的基因的浓度，对来自西澳大利亚的90种园林土壤的抗生素抗性基因进行了检测，以评估总金属对抗生素抗性基因的影响情况。金属和基因之间存在关系，但趋势各不相同。除Se和Co外，所有金属都至少与一个AR基因在绝对基因数上相关，但只有Al、Mn和Pb与表现出抗性的较高百分比土壤细菌相关，这是种群选择的可能指标。多元线性回归模型中同时考虑多种因素时，相关系数有所提高，提示可能存在加性效应。在确定环境中AR的风险和抗性扩散时，必须考虑土壤金属浓度。

Increasing drug-resistant infections have drawn research interest towards examining environmental bacteria and the discovery that many factors, including elevated metal conditions, contribute to proliferation of antibiotic resistance (AR). This study examined 90 garden soils from Western Australia to evaluate predictions of antibiotic resistance genes from total metal conditions by comparing the concentrations of 12 metals and 13 genes related to tetracycline, beta-lactam and sulphonamide resistance. Relationships existed between metals and genes, but trends varied. All metals, except Se and Co, were related to at least one AR gene in terms of absolute gene numbers, but only Al, Mn and Pb were associated with a higher percentage of soil bacteria exhibiting resistance, which is a possible indicator of population selection. Correlations improved when multiple factors were considered simultaneously in a multiple linear regression model, suggesting the possibility of additive effects occurring. Soil-metal concentrations must be considered when determining risks of AR in the environment and the proliferation of resistance.

https://link.springer.com/article/10.1007/s11356-016-7997-y