摘要
     随着城市化和工业化的快速发展，城市周边地区往往成为垃圾堆放场，这可能加剧从垃圾到土壤细菌的抗生素耐药性的发生和传播。然而，抗生素耐药谱及影响其在城市周边地区传播的相关因素尚未得到充分研究。本文采用高通量qPCR技术，在流域尺度上对4个季节中城郊耕地和原始土壤的抗生素抗性基因( ARGs )进行了分析。城市周边土壤中的ARGs种类繁多，含量丰富，共检测到222个基因。与原始土壤相比，耕地土壤中含有更多不同的ARGs，并且几乎所有在原始土壤中检测到的ARGs也在耕地中检测到。随机森林预测表明，ARGs的整体模式与土地利用类型密切相关。mantel试验和部分冗余分析表明，细菌群落变异是抗生素耐药性变化的主要原因。ARGs丰度与可移动遗传因子( MGEs )呈显著正相关，提示ARGs在城市周边地区具有潜在的移动性。我们的结果扩展了对城市周边地区抗药性组成的认识，并提出人为活动推动了其空间和时间分布。

       With the rapid development of urbanization and industrialization, the peri-urban areas are often the sites for waste dumps, which may exacerbate the occurrence and spread of antibiotic resistance from waste to soil bacteria. However, the profiles of antibiotic resistomes and the associated factors influencing their dissemination in peri-urban areas have not been fully explored. Here, we characterized the antibiotic resistance genes (ARGs) in peri-urban arable and pristine soils in four seasons at the watershed scale, by using high-throughput qPCR. ARGs in peri-urban soils were diverse and abundant, with a total of 222 genes were detected in the peri-urban soil samples. The arable soil harbored more diverse ARGs compared to the pristine soils, and nearly all the ARGs detected in the pristine soils were also detected in the farmlands. A random forest prediction showed that the overall patterns of ARGs clustered closely with the landuse type. Mantel test and partial redundancy analysis indicated that bacterial community variation is a major contributor to antibiotic resistome alteration. Significant positive correlation was found between the abundance of ARGs and mobile genetic elements (MGEs), suggesting potential mobility of ARGs in peri-urban areas. Our results extend knowledge of the resistomes compositions in peri-urban areas, and suggest that anthropogenic activities driving its spatial and temporal distribution.
 
https://www.sciencedirect.com/science/article/pii/S026974911734407X