摘要

       调查了从中国深圳主要河流采集的地表水和沉积物中抗生素的发生和分布。 31 个水样中 20 种选定抗生素的总浓度范围为 36.510 至 1075.687ngL-1（平均 244.992ngL-1），31 个沉积物样品中的总浓度为 28.124 至 2728.810ngg-1（平均 680.169ngL-1）。值得注意的是，STZ 和 SDZ 是水和沉积物中的主要抗生素，因为与其他化合物相比，它们的浓度更高。此外，对抗生素抗性基因（ARGs）和微生物群落进行了综合分析，以了解由于茅洲河沉积物样品中抗生素的出现而引起的微生物群落中ARGs的进化和传播。结果，发现 sul1 基因是最丰富的 ARG，变形杆菌是所有样品中最丰富的微生物（37.4-51.7%），其次是拟杆菌（15.3-18.4%）。统计分析找出了抗生素、ARGs和微生物群落之间的关系。从bla_d基因、梭杆菌和磺胺甲恶唑之间的正相关可以得出一个具体的结论。这表明抗生素可能与某些细菌中 ARGs 的表达呈正相关，因此细菌群落内会发生高繁殖。总体而言，ARG 的广泛分布强调需要进一步研究抗生素作为环境中新兴污染物的影响机制以及对微生物群落的相关风险。

       The occurrence and distribution of antibiotics were investigated in surface water and sediment collected from the main rivers of Shenzhen, China. Total concentrations of 20 selected antibiotics ranged from 36.510 to 1075.687 ng L−1 (mean 244.992 ng L−1) in 31 water samples and from 28.124 to 2728.810 ng g−1 (mean 680.169 ng g−1) in 31 sediment samples. Notably, STZ and SDZ were the dominant antibiotics in both water and sediment as their higher concentrations compared with the other compounds. Furthermore, comprehensive profiling of antibiotic resistance genes (ARGs) and microbial community was performed to gain an understanding of the evolution and dissemination of ARGs in microbial communities caused by the occurrence of antibiotics in sediment samples from Maozhou River. As a result, the sul1 gene was found to be the most abundant ARG and Proteobacteria was the most abundant microorganism in all the samples (37.4–51.7%), followed by Bacteroidetes (15.3–18.4%). Statistical analysis figured out the relations among antibiotics, ARGs and microbial community. A specific conclusion could be drawn from the positive correlations among the bla_d gene, Fusobacteria, and sulfamethoxazole. It suggests that antibiotics may be positively linked to the expression of ARGs in certain bacteria, and thus high reproduction would occur within the bacterial community. Overall, the widespread distribution of ARGs underscores the need for further research on the mechanism of antibiotics influence as emerging contaminants in the environment and the associated risks to microbial community.

https://www.sciencedirect.com/science/article/abs/pii/S0048969718342980