摘要

       迄今为止尚未注意到的风险来自使用非抗生素。 在这项研究中，非抗生素药物（环丙氨嗪）被用于堆肥，以研究其对 ARGs 分布和细菌群落变化的可能影响。 结果表明，环丙氨嗪增加了高风险 ARG（blaCTX-M 和 blaVIM）和重金属抗性基因 (MRG) 的丰度。 与对照处理相比，低浓度和高浓度的环丙氨嗪使 Tn916/1545 的丰度分别增加了 18.27% 和 64.26%。 移动遗传元素（MGEs）和MRGs不是ARGs动态变化的主要原因，而是细菌群落演替根据水分含量、pH值和生物铜发生变化。 网络分析表明变形菌和放线菌是ARGs的主要宿主，tcrB、sul1和Tn916/1545之间存在显着相关性。 

       The risks that have not been noted so far have come from the use of non-antibiotics. In this study, non-antibiotic drug (cyromazine) was used in composting to investigate its possible effects on the distribution of ARGs and changes of bacterial community. Results showed that cyromazine increased the abundances of highly-risky ARGs (blaCTX-M and blaVIM), and heavy metal resistance genes (MRGs). Low and high concentrations of cyromazine increased the abundance of Tn916/1545 by 18.27% and 64.26%, respectively, compared with the control treatment. Mobile genetic elements (MGEs) and MRGs were not the major cause of the dynamic changes in ARGs, but instead the bacterial community succession changed according to the moisture content, pH, and bio-Cu. Network analysis showed that Proteobacteria and Actinobacteria were the major hosts for ARGs, and there was a significant correlation between tcrB, sul1 and Tn916/1545.

https://www.sciencedirect.com/science/article/abs/pii/S096085241831647X