摘要

       本研究建立了两个中温厌氧消化器，以确定高浓度抗生素污泥厌氧消化（AD）过程中抗生素抗性基因（ARGs）的微生物动力学和变异特征。分析了系统参数、微生物群落、ARG（tetA、tetM、tetW、sulI、sulII）和 1 类整合酶基因（intI1）。 AD的总体表现表明，在抗生素的压力下，甲烷的产生被抑制了17.1%。微生物16S rRNA高通量测序结果显示微生物群落的丰富度下降，但添加抗生素后发现多样性更高。此外，属水平的微生物群落结构发生了显着变化。几个靶基因的实时定量 PCR 表明，高浓度抗生素的加入对 ARG 产生了显着的诱导影响，然而，intI1 的丰度明显下降。相关分析表明，intI1在AD期间ARGs的转移中只发挥了很小的作用，潜在宿主的变化是关键因素。

       This study established two mesophilic anaerobic digesters to ascertain the microbial dynamics and variation characteristics of antibiotic resistance genes (ARGs) during sludge anaerobic digestion (AD) with high concentration of antibiotics. System parameters, microbial community, ARGs (tetA, tetM, tetW, sulI, sulII) and integrase gene of class 1 (intI1) were analyzed. General performance of AD showed methane production was inhibited by 17.1% under the pressure of antibiotics. Microbial 16S rRNA high-throughput sequencing results showed the richness of microbial community decreased, but a higher diversity was found with antibiotics added. Furthermore, microbial community structure at genus level was significantly changed. Real-time quantitative PCR of several target genes demonstrated that the adjunction of high concentration of antibiotics exerted a significant induction influence on ARGs, however, the abundance of intI1 decreased observably. Correlation analysis showed intI1 only played a small role in ARGs’ transfer during AD, change of potential hosts was the key factor instead.

https://www.sciencedirect.com/science/article/abs/pii/S0960852418317346