摘要

       公众越来越关注废水处理过程中抗生素抗性基因 (ARG) 的归宿、它们在处理过程中的持久性及其对接收水体的潜在影响。在这项研究中，我们使用定量 PCR (qPCR) 来确定来自 10 个不同欧洲国家的 16 个污水处理厂 (WWTP) 流出物中 9 个 ARG 和 1 类整合子相关整合酶基因的丰度。为了评估对受纳水体的影响，还分析了后者的基因丰度。
在所有污水和河水样品中检测到分析的九个 ARG 中的六个。在量化的基因中，intI1 和 sul1 是最丰富的。我们的结果表明，欧洲污水处理厂有助于富集受纳水体中的抗性组，其特定影响取决于污水负荷和当地水文条件。发现污水处理厂流出物中的 ARGs 浓度与实施的生物处理步骤的数量成反比，表明污水处理厂管理的可能选择。
此外，这项研究已将 blaOXA-58 确定为未来研究污水处理厂对其接收水影响的可能的抗性基因。

       There is increasing public concern regarding the fate of antibiotic resistance genes (ARGs) during wastewater treatment, their persistence during the treatment process and their potential impacts on the receiving water bodies. In this study, we used quantitative PCR (qPCR) to determine the abundance of nine ARGs and a class 1 integron associated integrase gene in 16 wastewater treatment plant (WWTP) effluents from ten different European countries. In order to assess the impact on the receiving water bodies, gene abundances in the latter were also analysed.
Six out of the nine ARGs analysed were detected in all effluent and river water samples. Among the quantified genes, intI1 and sul1 were the most abundant. Our results demonstrate that European WWTP contribute to the enrichment of the resistome in the receiving water bodies with the particular impact being dependent on the effluent load and local hydrological conditions. The ARGs concentrations in WWTP effluents were found to be inversely correlated to the number of implemented biological treatment steps, indicating a possible option for WWTP management.
Furthermore, this study has identified blaOXA-58 as a possible resistance gene for future studies investigating the impact of WWTPs on their receiving water.

https://www.sciencedirect.com/science/article/pii/S0043135419305524