摘要

       水生环境中抗生素抗性基因 (ARG) 的命运，尤其是河流和水库，在韩国受到越来越多的关注，因为水库是该国重要的饮用水源。对位于天安市和清州市饮用水处理厂附近的韩国两个水库中 11 个 ARG 和一个移动遗传元件 (int1) 丰度的季节性变化进行了 6 个月的监测。在这些饮用水源中，总 ARG 浓度达到 2.5 × 107 拷贝 mL-1，比韩国一些污水处理厂的进水高一个数量级。在2016年8月、10月、11月和2017年1月的采样期间，磺胺类（sul1）、β-内酰胺类抗生素（blaTEM）和四环素（tetA）抗性基因是两个位点最丰富的基因。相对于 16S 核糖体核糖核酸 (rRNA) 计数，ARG 丰度在 1 月份持续增加。对氧化应激和与寒冷季节相关的其他环境因素的一般应激反应可能是环境中 ARG 水平基因转移的重要驱动因素。因此，去除 ARG 作为水处理的关键步骤值得更多关注。

       The fate of antibiotic resistance genes (ARGs) in aquatic environments, especially in rivers and reservoirs, is receiving growing attention in South Korea because reservoirs are an important source of drinking water in this country. Seasonal changes in the abundance of 11 ARGs and a mobile genetic element (int1) in two reservoirs in South Korea, located near drinking water treatment plants in Cheonan and Cheongju cities, were monitored for 6 mo. In these drinking water sources, total ARG concentrations reached 2.5 × 107 copies mL‒1, which is one order of magnitude higher than in influents of some wastewater treatment plants in South Korea. During the sampling periods in August, October, and November 2016 and January 2017, sulfonamides (sul1), β-lactam antibiotics (blaTEM), and tetracycline (tetA) resistance genes were the most abundant genes at the two sites. The ARG abundance consistently increased in January relative to 16S ribosomal ribonucleic acid (rRNA) counts. General stress responses to oxidative stress and other environmental factors associated with the cold season could be significant drivers of ARG horizontal gene transfer in the environment. Accordingly, removal of ARGs as a key step in water treatment warrants more attention.

https://acsess.onlinelibrary.wiley.com/doi/abs/10.2134/jeq2017.12.0493