摘要

       猪粪是抗生素抗性基因 (ARG) 的储存库，可能会从养猪场传播到环境中。本研究建立了用于去除猪废水中ARG的序批式膜生物反应器（SMBR），并分析了生物处理和膜分离对不同固体保留时间（SRT）下ARG去除的影响。 SMBR 在短 SRT（12 天）中删除了 2.91 个日志（拷贝数）的 ARG。提高 SRT 降低了生物处理检测到的基因的去除率。在相对较长的SRT（30天）下，ARGs和移动遗传元件（MGEs）在反应器内最大化，通过膜分离被很好地去除，平均基因去除率为2.95（拷贝数）和1.18logs（丰度）。在相对较低的 SRT 下，生物处理显示出占主导地位的 ARG 去除效果，而膜分离则发挥了去除 ARG 的优势，尤其是在相对较长的 SRT 下。 ARG 谱与微生物群落结构的变化有关。 ARGs 与功能细菌（氨氧化细菌、亚硝酸盐氧化细菌和反硝化细菌）共存，表明它们由功能细菌寄主。

       Swine wastes are the reservoir of antibiotic resistance genes (ARGs), which can potentially spread from swine farms to the environment. This study establishes a sequencing-batch membrane bioreactor (SMBR) for ARG removal from swine wastewater, and analyzes the effect of biological treatment and membrane separation on the ARG removal at different solid retention times (SRTs). The SMBR removed 2.91 logs (copy number) of ARGs at a short SRT (12 days). Raising the SRT reduced the removal rates of the detected genes by the biological treatment. Under the relative long SRT (30 days), ARGs and mobile genetic elements (MGEs) were maximized within the reactor and were well removed by membrane separation, with the average genes removal rate of 2.95 (copy number) and 1.18 logs (abundance). At the relatively low SRT, the biological treatment showed the dominant ARG removal effect, while the membrane separation took the advantages of ARG removal especially at the relatively long SRT. The ARG profile was related to the shift of the microbial community structure. The ARGs coexisted with the functional bacteria (ammonia oxidizing bacteria, nitrite oxidizing bacteria and denitrifiers), suggesting they are hosted by the functional bacteria.

https://www.sciencedirect.com/science/article/pii/S0160412018301788