摘要

       微生物燃料电池耦合人工湿地 (CW-MFC) 使用电化学、生物和生态功能来处理废水。然而，很少有研究调查使用此类系统去除抗生素时抗生素抗性基因 (ARG) 的风险。因此，设计了三个 CW-MFC 来评估填料生物膜和流出物中 ARG 的动态超过 5000 小时的运行。实验结果表明，在总进水抗生素浓度分别为 400、1,000 和 1600 μg L−1 时，获得了相对较高的稳态电压，分别为 605.8 mV、613.7 mV 和 541.4 mV。阴极层的 16S rRNA 基因水平高于阳极层和两个中间层，但 sul 和 tet 基因的趋势相反。三个被测sul基因的相对丰度顺序为sulI>sulII>sulIII，五个tet基因的相对丰度顺序为tetA>tetC>tetW>tetO>tetQ。培养基生物膜中 sul 和 tet 基因的水平在治疗期间显示出增加。与填料生物膜相比，出水的 sul 和 tet 基因丰度相对较低。在处理期间没有观察到大多数 ARGs 增加，并且除了流出物中的 sulI 和 tetW 外，ARGs 和 16S rRNA 基因拷贝数之间没有观察到显着相关性。然而，在大多数 ARG 拷贝数之间观察到了显着的相关性。

       Microbial fuel cell-coupled constructed wetlands (CW-MFCs) use electrochemical, biological, and ecological functions to treat wastewater. However, few studies have investigated the risks of antibiotic resistance genes (ARGs) when using such systems to remove antibiotics. Therefore, three CW-MFCs were designed to assess the dynamics of ARGs in filler biofilm and effluent over 5000 h of operation. The experimental results indicated that relatively high steady voltages of 605.8 mV, 613.7 mV, and 541.4 mV were obtained at total influent antibiotic concentrations of 400, 1,000, and 1600 μg L−1, respectively. The 16S rRNA gene level in the cathode layer was higher than those in the anode and two middle layers, but the opposite trend was observed for the sul and tet genes. The relative abundance of the three tested sul genes were in the order sulI > sulII > sulIII, and those of the five tet genes were in the order tetA > tetC > tetW > tetO > tetQ. The levels of sul and tet genes in the media biofilm showed an increase over the treatment period. The effluent water had relatively low abundances of sul and tet genes compared with the filler biofilm. No increases were observed for most ARGs over the treatment period, and no significant correlations were observed between the ARGs and 16S rRNA gene copy numbers, except for sulI and tetW in the effluent. However, significant correlations were observed among most of the ARG copy numbers.

https://www.sciencedirect.com/science/article/abs/pii/S0045653517304599