摘要

       水平基因转移 (HGT) 在抗生素抗性基因 (ARG) 在环境中的传播中起着重要作用。 然而，在亚 MIC 中抗生素影响下 ARGs 的 HGT 机制仍然很少被探索。 此外，鉴于其集体性质，HGT 被认为与群体感应 (QS) 系统相关。 为了研究 QS 是否对 ARG 的水平基因转移有任何影响，进行了实验以确定质粒 RP4 在四环素 (TC)、群体感应自诱导剂 (AI) 和群体影响下对大肠杆菌 (E.coli) 的接合效率。 传感抑制剂（QSIs）。 结果表明亚MIC TCs可以促进RP4的结合转移，这一过程可以被AIs增强但被QSIs抑制。 该研究证明了 QS 在 ARGs 传播中的作用，并为 ARGs 在环境中的 HGT 机制提供了理论见解。 

       Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E.coli) under the influences of tetracyclines (TCs), quorum sensing autoinducers (AIs) and quorum sensing inhibitors (QSIs). The results indicated that the sub-MIC TCs could facilitate the conjugative transfer of RP4, a process which could be enhanced by AIs but inhibited by QSIs. This study demonstrated the roles that QS played in the dissemination of ARGs, and provided theoretical insights into the mechanism of HGT of ARGs in the environment.

https://link.springer.com/article/10.1007/s10646-017-1886-0