摘要

       对来自中国北京的六个使用再生水的公园土壤中抗生素和抗生素抗性基因 (ARG) 的丰度和分布进行了表征。通过液相色谱-串联质谱 (LC-MS/MS) 分析了三类常用抗生素（四环类、喹诺酮类和磺胺类）。四环素类和喹诺酮类的最高浓度分别为 145.2 μg kg-1 和 79.2 μg kg-1。通过定量PCR对检测到的tetG、tetW、sulI和sulII基因进行定量。对于不同的公园土壤，ARGs 表现出不同的丰度。整合酶基因（intI1）作为水平基因转移潜力的指标也被检测到高丰度，并且与tetG、sulI和sulII基因呈显着正相关，表明intI1可能参与了ARGs的传播。 sulII 和 intI1 克隆与某些类别的病原菌具有高度同源性，如克雷伯氏菌、鲍曼不动杆菌、弗氏志贺氏菌，可能引发潜在的公共卫生问题。

       The abundance and distribution of antibiotics and antibiotic resistance genes (ARGs) in soils from six parks using reclaimed water in Beijing, China, were characterized. Three classes of commonly used antibiotics (tetracycles, quinolones, and sulfonamides) were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The highest concentrations of tetracyclines and quinolones were 145.2 μg kg−1 and 79.2 μg kg−1, respectively. Detected tetG, tetW, sulI, and sulII genes were quantified by quantitative PCR. ARGs exhibited various abundances for different park soils. The integrase gene (intI1) as an indicator of horizontal gene transfer potential was also detected in high abundance, and had significant positive correlation with tetG, sulI, and sulII genes, suggesting that intI1 may be involved in ARGs dissemination. Both sulII and intI1 clones had high homology with some classes of pathogenic bacteria, such as Klebsiella oxytoca, Acinetobacter baumannii, Shigella flexneri, which could trigger potential public health concern.

https://www.sciencedirect.com/science/article/abs/pii/S026974911300465X