摘要

       城市污水处理厂 (WWTP) 流出物是抗生素耐药性的重要污染源，威胁着接收环境的生态安全。本研究调查了中国 10 座污水处理厂废水中磺胺类和四环素类抗生素耐药性的释放情况。结果表明，抗生素抗性细菌 (ARB) 和抗生素抗性基因 (ARG) 的浓度范围为 1.1 × 101 至 8.9 × 103 CFU mL−1 和 3.6 × 101 (tetW) 至 5.4 × 106 (tetX) 拷贝 mL− 1、分别。 ARB和ARGs的浓度与相应抗生素的浓度无显着相关性。在四环素抗性基因和磺胺类抗性基因的总浓度之间观察到很强的相关性，并且两者都与 intI1 浓度显着相关。对不同污水处理厂出水 ARG 浓度的统计分析揭示了消毒在消除抗生素耐药性方面的重要作用。 ARB 和 ARGs 通过 10 个污水处理厂出水的释放速率范围分别为 5.9 × 1012 至 4.8 × 1015 CFU d-1 和 6.4 × 1012 (tetW) 至 1.7 × 1018 (sul1) 拷贝 d-1。本研究有助于对污水处理厂排放抗生素耐药性引起的生态风险进行有效评估和科学管理。

       Municipal wastewater treatment plant (WWTP) effluents represent an important contamination source of antibiotic resistance, threatening the ecological safety of receiving environments. In this study, the release of antibiotic resistance to sulfonamides and tetracyclines in the effluents of ten WWTPs in China was investigated. Results indicate that the concentrations of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) ranged from 1.1 × 101 to 8.9 × 103 CFU mL−1 and 3.6 × 101 (tetW) to 5.4 × 106 (tetX) copies mL−1, respectively. There were insignificant correlations of the concentrations of ARB and ARGs with those of corresponding antibiotics. Strong correlations were observed between the total concentrations of tetracycline resistance genes and sulfonamide resistance genes, and both of which were significantly correlated with intI1 concentrations. Statistical analysis of the effluent ARG concentrations in different WWTPs revealed an important role of disinfection in eliminating antibiotic resistance. The release rates of ARB and ARGs through the effluents of ten WWTPs ranged from 5.9 × 1012 to 4.8 × 1015 CFU d−1 and 6.4 × 1012 (tetW) to 1.7 × 1018 (sul1) copies d−1, respectively. This study helps the effective assessment and scientific management of ecological risks induced by antibiotic resistance discharged from WWTPs.

https://www.sciencedirect.com/science/article/abs/pii/S0045653517300437