摘要

       硝酸盐和抗生素/抗生素抗性基因 (ARG) 的混合污染是农田土壤新出现的环境风险。 这是第一项探讨过量人为硝酸盐输入在土壤抗生素/ARGs 缺氧消散中的作用的研究。 在孵化的最初 10 天，土壤抗生素的存在显着抑制了土壤中 NO3− 耗散、N2O 生成率和反硝化基因 (DNG) 丰度（p < 0.05）。 相比之下，在第 10 天和第 30 天之间，反硝化作用增强明显促使抗生素含量和 ARG 丰度下降。 在 DNG 和 ARG 之间检测到显着的负相关，表明 DNG 活性越高，反硝化作用越显着，抗生素耗散和 ARG 丰度越大。 这项研究为理解在增强型缺氧反硝化条件下土壤 DNG 和 ARG 之间的相互作用提供了重要知识。 

       Mixed contamination of nitrate and antibiotics/antibiotic-resistant genes (ARGs) is an emerging environmental risk to farmland soil. This is the first study to explore the role of excessive anthropogenic nitrate input in the anoxic dissipation of soil antibiotic/ARGs. During the initial 10 days of incubation, the presence of soil antibiotics significantly inhibited NO3− dissipation, N2O production rate, and denitrifying genes (DNGs) abundance in soil (p < 0.05). Between days 10 and 30, by contrast, enhanced denitrification clearly prompted the decline in antibiotic contents and ARG abundance. Significantly negative correlations were detected between DNGs and ARGs, suggesting that the higher the DNG activity, the more dramatic is the denitrification and the greater are the antibiotic dissipation and ARG abundance. This study provides crucial knowledge for understanding the mutual interaction between soil DNGs and ARGs in the enhanced anoxic denitrification condition.

https://www.sciencedirect.com/science/article/abs/pii/S0269749116316463