摘要

       本研究旨在确定生态农业系统（EAS）中抗生素和抗生素抗性基因（ARGs）的丰度和持久性，从猪粪到厌氧消化产物，然后应用厌氧消化固体残留物（ADSR）和厌氧消化液残留物 (ADLR) 到土壤中种植黑麦草，一种猪饲料。土霉素在粪便中的浓度最高，达到 138.7 mg/kg。厌氧消化可以有效去除大部分抗生素，去除率从11%到86%不等。 ARGs 丰度在 EAS 内波动。 TetQ 的相对丰度最高，tetG 的相对丰度在系统内变异最小，说明 tetG 在农业环境中具有持久性，需要引起更多关注。与粪便中的相对丰度相比，沼渣中的tetC和tetM增加，而三个核糖体保护蛋白基因（tetO、tetQ、tetW）减少（p < 0.05），其他基因在厌氧发酵后无显着变化（p > 0.05） .除 tetG 和 sulI 外，EAS 下游组件（土壤和鱼塘）中的大多数 ARG 显示出比对照农业系统显着更高的相对丰度（p < 0.05）。

       This study aims to determine abundance and persistence of antibiotics and antibiotic resistance genes (ARGs) in eco-agricultural system (EAS), which starts from swine feces to anaerobic digestion products, then application of anaerobic digestion solid residue (ADSR) and anaerobic digestion liquid residue (ADLR) to the soil to grow ryegrass, one of swine feed. Oxytetracycline had the highest concentration in manure reaching up to 138.7 mg/kg. Most of antibiotics could be effectively eliminated by anaerobic digestion and removal rates ranged from 11% to 86%. ARGs abundance fluctuated within EAS. TetQ had the highest relative abundance and the relative abundance of tetG had the least variation within the system, which indicates that tetG is persistent in the agricultural environment and requires more attention. Compared to the relative abundance in manure, tetC and tetM increased in biogas residue while three ribosomal protection proteins genes (tetO, tetQ, tetW) decreased (p < 0.05), with other genes showing no significant change after anaerobic fermentation (p > 0.05). Most ARGs in downstream components (soils and fishpond) of EAS showed significantly higher relative abundance than the control agricultural system (p < 0.05), except for tetG and sulI.

https://www.sciencedirect.com/science/article/abs/pii/S0304389415301643